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@article{2357976,
author = {Tatíčková, Martina and Trebichalská, Zuzana and Kyjovská, Drahomíra and Otevřel, Pavel and Kloudová, Soňa and Holubcová, Zuzana},
article_location = {New York},
article_number = {4},
doi = {http://dx.doi.org/10.1016/j.xfss.2023.09.002},
keywords = {Cytoskeleton; dysmorphism; electron microscopy; human oocyte; oocyte abnormalities},
language = {eng},
issn = {2666-335X},
journal = {F&S science},
title = {The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction},
url = {https://www.sciencedirect.com/science/article/pii/S2666335X23000502?via%3Dihub},
volume = {4},
year = {2023}
}
TY - JOUR
ID - 2357976
AU - Tatíčková, Martina - Trebichalská, Zuzana - Kyjovská, Drahomíra - Otevřel, Pavel - Kloudová, Soňa - Holubcová, Zuzana
PY - 2023
TI - The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction
JF - F&S science
VL - 4
IS - 4
SP - 267-278
EP - 267-278
PB - Elsevier
SN - 2666335X
KW - Cytoskeleton
KW - dysmorphism
KW - electron microscopy
KW - human oocyte
KW - oocyte abnormalities
UR - https://www.sciencedirect.com/science/article/pii/S2666335X23000502?via%3Dihub
N2 - Objective: To investigate the structural bases of human oocytes' cytoplasmic abnormalities and the causative mechanism of their emergence. Knowledge of an abnormal oocyte's intracellular organization is vital to establishing reliable criteria for clinical evaluation of oocyte morphology. Design: Laboratory-based study on experimental material provided by a private assisted reproduction clinic. Setting: University laboratory and imaging center. Patients: A total of 105 women undergoing hormonal stimulation for in vitro fertilization (IVF) donated their spare oocytes for this study. Interventions: Transmission electron microscopy (TEM) was used to analyze the fine morphology of 22 dysmorphic IVF oocytes exhibiting different types of cytoplasmic irregularities, namely, refractile bodies; centrally located cytoplasmic granularity (CLCG); smooth endoplasmic reticulum (SER) disc; and vacuoles. A total of 133 immature oocytes were exposed to cytoskeleton-targeting compounds or matured in control conditions, and their morphology was examined using fluorescent and electron microscopy. Main outcome measures: The ultrastructural morphology of dysmorphic oocytes was analyzed. Drug-treated oocytes had their maturation efficiency, chromosome-microtubule configurations, and fine intracellular morphology examined. Results: TEM revealed ultrastructural characteristics of common oocyte aberrations and indicated that excessive organelle clustering was the underlying cause of 2 of the studied morphotypes. Inhibition experiments showed that disruption of actin, not microtubules, allows for inordinate aggregation of subcellular structures, resembling the ultrastructural pattern seen in morphologically abnormal oocytes retrieved in IVF cycles. These results imply that actin serves as a regulator of organelle distribution during human oocyte maturation. Conclusion: The ultrastructural analogy between dysmorphic oocytes and oocytes, in which actin network integrity was perturbed, suggests that dysfunction of the actin cytoskeleton might be implicated in generating common cytoplasmic aberrations. Knowledge of human oocytes' inner workings and the origin of morphological abnormalities is a step forward to a more objective oocyte quality assessment in IVF practice.
ER -
TATÍČKOVÁ, Martina, Zuzana TREBICHALSKÁ, Drahomíra KYJOVSKÁ, Pavel OTEVŘEL, Soňa KLOUDOVÁ and Zuzana HOLUBCOVÁ. The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction. \textit{F\&{}S science}. New York: Elsevier, 2023, vol.~4, No~4, p.~267-278. ISSN~2666-335X. Available from: https://dx.doi.org/10.1016/j.xfss.2023.09.002.
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