Luminal Surface Plasma Treatment of Closed Cylindrical
Microchannels: A Tool toward the Creation of On-Chip Vascular
Endothelium

J 2023

Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium

ČERNÍK, Marek, Kamila POLAKOVA, Lukas KUBALA, Andrea VÍTEČKOVÁ WÜNSCHOVÁ, Anna MAC GILLAVRY DANYLEVSKA et. al.

Basic information

Original name

Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium

Authors

ČERNÍK, Marek (203 Czech Republic, belonging to the institution), Kamila POLAKOVA (203 Czech Republic), Lukas KUBALA (203 Czech Republic), Andrea VÍTEČKOVÁ WÜNSCHOVÁ (203 Czech Republic, belonging to the institution), Anna MAC GILLAVRY DANYLEVSKA (804 Ukraine, belonging to the institution), Michaela PEŠKOVÁ (203 Czech Republic, belonging to the institution) and Jan VÍTEČEK (guarantor)

Edition

ACS Biomaterials Science and Engineering, American Chemical Society, 2023, 2373-9878

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30401 Health-related biotechnology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.800 in 2022

RIV identification code

RIV/00216224:14310/23:00132874

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acsbiomaterials.2c00887

UT WoS

000982471500001

Keywords in English

3D printing; endothelial cell; in vitro model; plasma oxidation; PDMS; surface modification

Abstract

V originále

On-chip vascular microfluidic models provide a great tool to study aspects of cardiovascular diseases in vitro. To produce such models, polydimethylsiloxane (PDMS) has been the most widely used material. For biological applications, its hydrophobic surface has to be modified. The major approach has been plasma-based surface oxidation, which has been very challenging in the case of channels enclosed within a microfluidic chip. The preparation of the chip combined a 3D-printed mold with soft lithography and commonly available materials. We have introduced the high-frequency low-pressure air-plasma surface modification of seamless channels enclosed within a PDMS microfluidic chip. The plasma treatment modified the luminal surface more uniformly than in previous works. Such a setup enabled a higher degree of design freedom and a possibility of rapid prototyping. Further, plasma treatment in combination with collagen IV coating created a biomimetic surface for efficient adhesion of vascular endothelial cells as well as promoted long-term cell culture stability under flow. The cells within the channels were highly viable and showed physiological behavior, confirming the benefit of the presented surface modification.

Links

NU22-08-00124, research and development project

Name: Modelování toku v intrakraniálních cévách ve vztahu ke změnám endotelu a rozvoji intrakraniálních aneuryzmat

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

ČERNÍK, Marek, Kamila POLAKOVA, Lukas KUBALA, Andrea VÍTEČKOVÁ WÜNSCHOVÁ, Anna MAC GILLAVRY DANYLEVSKA, Michaela PEŠKOVÁ and Jan VÍTEČEK. Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium. ACS Biomaterials Science and Engineering. American Chemical Society, 2023, vol. 9, No 5, p. 2755-2763. ISSN 2373-9878. Available from: https://dx.doi.org/10.1021/acsbiomaterials.2c00887.

@article{2358468,
   author = {Černík, Marek and Polakova, Kamila and Kubala, Lukas and Vítečková Wünschová, Andrea and Mac Gillavry Danylevska, Anna and Pešková, Michaela and Víteček, Jan},
   article_number = {5},
   doi = {http://dx.doi.org/10.1021/acsbiomaterials.2c00887},
   keywords = {3D printing; endothelial cell; in vitro model; plasma oxidation; PDMS; surface modification},
   language = {eng},
   issn = {2373-9878},
   journal = {ACS Biomaterials Science and Engineering},
   title = {Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium},
   url = {https://doi.org/10.1021/acsbiomaterials.2c00887},
   volume = {9},
   year = {2023}
}

TY  - JOUR
ID  - 2358468
AU  - Černík, Marek - Polakova, Kamila - Kubala, Lukas - Vítečková Wünschová, Andrea - Mac Gillavry Danylevska, Anna - Pešková, Michaela - Víteček, Jan
PY  - 2023
TI  - Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium
JF  - ACS Biomaterials Science and Engineering
VL  - 9
IS  - 5
SP  - 2755-2763
EP  - 2755-2763
PB  - American Chemical Society
SN  - 23739878
KW  - 3D printing
KW  - endothelial cell
KW  - in vitro model
KW  - plasma oxidation
KW  - PDMS
KW  - surface modification
UR  - https://doi.org/10.1021/acsbiomaterials.2c00887
N2  - On-chip vascular microfluidic models provide a great tool to study aspects of cardiovascular diseases in vitro. To produce such models, polydimethylsiloxane (PDMS) has been the most widely used material. For biological applications, its hydrophobic surface has to be modified. The major approach has been plasma-based surface oxidation, which has been very challenging in the case of channels enclosed within a microfluidic chip. The preparation of the chip combined a 3D-printed mold with soft lithography and commonly available materials. We have introduced the high-frequency low-pressure air-plasma surface modification of seamless channels enclosed within a PDMS microfluidic chip. The plasma treatment modified the luminal surface more uniformly than in previous works. Such a setup enabled a higher degree of design freedom and a possibility of rapid prototyping. Further, plasma treatment in combination with collagen IV coating created a biomimetic surface for efficient adhesion of vascular endothelial cells as well as promoted long-term cell culture stability under flow. The cells within the channels were highly viable and showed physiological behavior, confirming the benefit of the presented surface modification.
ER  -

ČERNÍK, Marek, Kamila POLAKOVA, Lukas KUBALA, Andrea VÍTEČKOVÁ WÜNSCHOVÁ, Anna MAC GILLAVRY DANYLEVSKA, Michaela PEŠKOVÁ and Jan VÍTEČEK. Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium. \textit{ACS Biomaterials Science and Engineering}. American Chemical Society, 2023, vol.~9, No~5, p.~2755-2763. ISSN~2373-9878. Available from: https://dx.doi.org/10.1021/acsbiomaterials.2c00887.

Detailed Information on Publication Record