| ČERNÍK, Marek, Kamila POLAKOVA, Lukas KUBALA, Andrea VÍTEČKOVÁ WÜNSCHOVÁ, Anna MAC GILLAVRY DANYLEVSKA, Michaela PEŠKOVÁ and Jan VÍTEČEK. Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium. ACS Biomaterials Science and Engineering. American Chemical Society, 2023, vol. 9, No 5, p. 2755-2763. ISSN 2373-9878. Available from: https://dx.doi.org/10.1021/acsbiomaterials.2c00887. |
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@article{2358468,
author = {Černík, Marek and Polakova, Kamila and Kubala, Lukas and Vítečková Wünschová, Andrea and Mac Gillavry Danylevska, Anna and Pešková, Michaela and Víteček, Jan},
article_number = {5},
doi = {http://dx.doi.org/10.1021/acsbiomaterials.2c00887},
keywords = {3D printing; endothelial cell; in vitro model; plasma oxidation; PDMS; surface modification},
language = {eng},
issn = {2373-9878},
journal = {ACS Biomaterials Science and Engineering},
title = {Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium},
url = {https://doi.org/10.1021/acsbiomaterials.2c00887},
volume = {9},
year = {2023}
}
TY - JOUR
ID - 2358468
AU - Černík, Marek - Polakova, Kamila - Kubala, Lukas - Vítečková Wünschová, Andrea - Mac Gillavry Danylevska, Anna - Pešková, Michaela - Víteček, Jan
PY - 2023
TI - Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium
JF - ACS Biomaterials Science and Engineering
VL - 9
IS - 5
SP - 2755-2763
EP - 2755-2763
PB - American Chemical Society
SN - 23739878
KW - 3D printing
KW - endothelial cell
KW - in vitro model
KW - plasma oxidation
KW - PDMS
KW - surface modification
UR - https://doi.org/10.1021/acsbiomaterials.2c00887
N2 - On-chip vascular microfluidic models provide a great tool to study aspects of cardiovascular diseases in vitro. To produce such models, polydimethylsiloxane (PDMS) has been the most widely used material. For biological applications, its hydrophobic surface has to be modified. The major approach has been plasma-based surface oxidation, which has been very challenging in the case of channels enclosed within a microfluidic chip. The preparation of the chip combined a 3D-printed mold with soft lithography and commonly available materials. We have introduced the high-frequency low-pressure air-plasma surface modification of seamless channels enclosed within a PDMS microfluidic chip. The plasma treatment modified the luminal surface more uniformly than in previous works. Such a setup enabled a higher degree of design freedom and a possibility of rapid prototyping. Further, plasma treatment in combination with collagen IV coating created a biomimetic surface for efficient adhesion of vascular endothelial cells as well as promoted long-term cell culture stability under flow. The cells within the channels were highly viable and showed physiological behavior, confirming the benefit of the presented surface modification.
ER -
ČERNÍK, Marek, Kamila POLAKOVA, Lukas KUBALA, Andrea VÍTEČKOVÁ WÜNSCHOVÁ, Anna MAC GILLAVRY DANYLEVSKA, Michaela PEŠKOVÁ and Jan VÍTEČEK. Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium. \textit{ACS Biomaterials Science and Engineering}. American Chemical Society, 2023, vol.~9, No~5, p.~2755-2763. ISSN~2373-9878. Available from: https://dx.doi.org/10.1021/acsbiomaterials.2c00887.
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