Completing the TRB family: newly characterized members show
ancient evolutionary origins and distinct localization, yet
similar interactions

J 2023

Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions

KUSOVÁ, Alžbeta, Lenka STEINBACHOVÁ, Tereza PŘEROVSKÁ, Lenka ZÁVESKÁ DRÁBKOVÁ, Jan PALEČEK et. al.

Basic information

Original name

Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions

Authors

KUSOVÁ, Alžbeta (703 Slovakia, belonging to the institution), Lenka STEINBACHOVÁ, Tereza PŘEROVSKÁ (203 Czech Republic, belonging to the institution), Lenka ZÁVESKÁ DRÁBKOVÁ, Jan PALEČEK (203 Czech Republic, belonging to the institution), Ahamed KHAN, Gabriela RIGÓOVÁ (703 Slovakia, belonging to the institution), Zuzana GADIOU, Claire JOURDAIN, Tino STRICKER, Daniel SCHUBERT, David HONYS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Molecular Biology, Springer, 2023, 0167-4412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.100 in 2022

RIV identification code

RIV/00216224:14310/23:00132876

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s11103-023-01348-2

UT WoS

000978294100001

Keywords in English

Telomere repeat binding; TRB; PRC2; PEAT; TERT; Telomeric

Abstract

V originále

Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoters (telo-boxes). They are also known to interact with several protein complexes: telomerase, Polycomb repressive complex 2 (PRC2) E(z) subunits and the PEAT complex (PWOs-EPCRs-ARIDs-TRBs). Here we characterize two novel members of the TRB family (TRB4 and TRB5). Our wide phylogenetic analyses have shown that TRB proteins evolved in the plant kingdom after the transition to a terrestrial habitat in Streptophyta, and consequently TRBs diversified in seed plants. TRB4-5 share common TRB motifs while differing in several others and seem to have an earlier phylogenetic origin than TRB1-3. Their common Myb-like domains bind long arrays of telomeric repeats in vitro, and we have determined the minimal recognition motif of all TRBs as one telo-box. Our data indicate that despite the distinct localization patterns of TRB1-3 and TRB4-5 in situ, all members of TRB family mutually interact and also bind to telomerase/PRC2/PEAT complexes. Additionally, we have detected novel interactions between TRB4-5 and EMF2 and VRN2, which are Su(z)12 subunits of PRC2.

Links

CZ.1.05/3.2.00/08.0144, interní kód MU

Name: CERIT Scientific Cloud (Acronym: CERIT - SC)

Investor: Ministry of Education, Youth and Sports of the CR, 3.2 Promotion and providing information on R&D results

ED3.2.00/08.0144, research and development project

Name: CERIT Scientific Cloud

GA21-15841S, research and development project

Name: Genová exprese v samčí zárodečné linii: regulační faktory a jejich responsivní elementy. (Acronym: Regulace gen. exprese v pylu.)

Investor: Czech Science Foundation

GX20-01331X, research and development project

Name: Biogeneze a evoluce telomerázy u rostlin

Investor: Czech Science Foundation

LM2010005, research and development project

Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

KUSOVÁ, Alžbeta, Lenka STEINBACHOVÁ, Tereza PŘEROVSKÁ, Lenka ZÁVESKÁ DRÁBKOVÁ, Jan PALEČEK, Ahamed KHAN, Gabriela RIGÓOVÁ, Zuzana GADIOU, Claire JOURDAIN, Tino STRICKER, Daniel SCHUBERT, David HONYS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions. Plant Molecular Biology. Springer, 2023, vol. 112, 1-2, p. 61-83. ISSN 0167-4412. Available from: https://dx.doi.org/10.1007/s11103-023-01348-2.

@article{2358472,
   author = {Kusová, Alžbeta and Steinbachová, Lenka and Přerovská, Tereza and Záveská Drábková, Lenka and Paleček, Jan and Khan, Ahamed and Rigóová, Gabriela and Gadiou, Zuzana and Jourdain, Claire and Stricker, Tino and Schubert, Daniel and Honys, David and Procházková Schrumpfová, Petra},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1007/s11103-023-01348-2},
   keywords = {Telomere repeat binding; TRB; PRC2; PEAT; TERT; Telomeric},
   language = {eng},
   issn = {0167-4412},
   journal = {Plant Molecular Biology},
   title = {Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions},
   url = {https://doi.org/10.1007/s11103-023-01348-2},
   volume = {112},
   year = {2023}
}

TY  - JOUR
ID  - 2358472
AU  - Kusová, Alžbeta - Steinbachová, Lenka - Přerovská, Tereza - Záveská Drábková, Lenka - Paleček, Jan - Khan, Ahamed - Rigóová, Gabriela - Gadiou, Zuzana - Jourdain, Claire - Stricker, Tino - Schubert, Daniel - Honys, David - Procházková Schrumpfová, Petra
PY  - 2023
TI  - Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions
JF  - Plant Molecular Biology
VL  - 112
IS  - 1-2
SP  - 61-83
EP  - 61-83
PB  - Springer
SN  - 01674412
KW  - Telomere repeat binding
KW  - TRB
KW  - PRC2
KW  - PEAT
KW  - TERT
KW  - Telomeric
UR  - https://doi.org/10.1007/s11103-023-01348-2
N2  - Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoters (telo-boxes). They are also known to interact with several protein complexes: telomerase, Polycomb repressive complex 2 (PRC2) E(z) subunits and the PEAT complex (PWOs-EPCRs-ARIDs-TRBs). Here we characterize two novel members of the TRB family (TRB4 and TRB5). Our wide phylogenetic analyses have shown that TRB proteins evolved in the plant kingdom after the transition to a terrestrial habitat in Streptophyta, and consequently TRBs diversified in seed plants. TRB4-5 share common TRB motifs while differing in several others and seem to have an earlier phylogenetic origin than TRB1-3. Their common Myb-like domains bind long arrays of telomeric repeats in vitro, and we have determined the minimal recognition motif of all TRBs as one telo-box. Our data indicate that despite the distinct localization patterns of TRB1-3 and TRB4-5 in situ, all members of TRB family mutually interact and also bind to telomerase/PRC2/PEAT complexes. Additionally, we have detected novel interactions between TRB4-5 and EMF2 and VRN2, which are Su(z)12 subunits of PRC2.
ER  -

KUSOVÁ, Alžbeta, Lenka STEINBACHOVÁ, Tereza PŘEROVSKÁ, Lenka ZÁVESKÁ DRÁBKOVÁ, Jan PALEČEK, Ahamed KHAN, Gabriela RIGÓOVÁ, Zuzana GADIOU, Claire JOURDAIN, Tino STRICKER, Daniel SCHUBERT, David HONYS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions. \textit{Plant Molecular Biology}. Springer, 2023, vol.~112, 1-2, p.~61-83. ISSN~0167-4412. Available from: https://dx.doi.org/10.1007/s11103-023-01348-2.

Detailed Information on Publication Record