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@article{2358577,
author = {Balakhonova, Veronika and Dobisová, Tereza and Benedikty, Zuzana and Panzarova, Klara and Pytela, Jaromir and Koci, Radka and Spyroglou, Ioannis and Kováčová, Ingrid and Arnaud, Dominique and Skalák, Jan and Trtilek, Martin and Hejátko, Jan},
article_number = {April},
doi = {http://dx.doi.org/10.3389/fpls.2023.1093292},
keywords = {de-etiolation; chlorophyll biosynthesis; fluorescence; iReenCAM; cytokinins; ethylene; Arabidopsis},
language = {eng},
issn = {1664-462X},
journal = {Frontiers in Plant Science},
title = {iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation},
url = {https://doi.org/10.3389/fpls.2023.1093292},
volume = {14},
year = {2023}
}
TY - JOUR
ID - 2358577
AU - Balakhonova, Veronika - Dobisová, Tereza - Benedikty, Zuzana - Panzarova, Klara - Pytela, Jaromir - Koci, Radka - Spyroglou, Ioannis - Kováčová, Ingrid - Arnaud, Dominique - Skalák, Jan - Trtilek, Martin - Hejátko, Jan
PY - 2023
TI - iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation
JF - Frontiers in Plant Science
VL - 14
IS - April
SP - 1-15
EP - 1-15
PB - Frontiers Media SA
SN - 1664462X
KW - de-etiolation
KW - chlorophyll biosynthesis
KW - fluorescence
KW - iReenCAM
KW - cytokinins
KW - ethylene
KW - Arabidopsis
UR - https://doi.org/10.3389/fpls.2023.1093292
N2 - Seedling de-etiolation is one of the key stages of the plant life cycle, characterized by a strong rearrangement of the plant development and metabolism. The conversion of dark accumulated protochlorophyllide to chlorophyll in etioplasts of de-etiolating plants is taking place in order of ns to mu s after seedlings illumination, leading to detectable increase of chlorophyll levels in order of minutes after de-etiolation initiation. The highly complex chlorophyll biosynthesis integrates number of regulatory events including light and hormonal signaling, thus making de-etiolation an ideal model to study the underlying molecular mechanisms. Here we introduce the iReenCAM, a novel tool designed for non-invasive fluorescence-based quantitation of early stages of chlorophyll biosynthesis during de-etiolation with high spatial and temporal resolution. iReenCAM comprises customized HW configuration and optimized SW packages, allowing synchronized automated measurement and analysis of the acquired fluorescence image data. Using the system and carefully optimized protocol, we show tight correlation between the iReenCAM monitored fluorescence and HPLC measured chlorophyll accumulation during first 4h of seedling de-etiolation in wild type Arabidopsis and mutants with disturbed chlorophyll biosynthesis. Using the approach, we demonstrate negative effect of exogenously applied cytokinins and ethylene on chlorophyll biosynthesis during early de-etiolation. Accordingly, we identify type-B response regulators, the cytokinin-responsive transcriptional activators ARR1 and ARR12 as negative regulators of early chlorophyll biosynthesis, while contrasting response was observed in case of EIN2 and EIN3, the components of canonical ethylene signaling cascade. Knowing that, we propose the use of iReenCAM as a new phenotyping tool, suitable for quantitative and robust characterization of the highly dynamic response of seedling de-etiolation.
ER -
BALAKHONOVA, Veronika, Tereza DOBISOVÁ, Zuzana BENEDIKTY, Klara PANZAROVA, Jaromir PYTELA, Radka KOCI, Ioannis SPYROGLOU, Ingrid KOVÁČOVÁ, Dominique ARNAUD, Jan SKALÁK, Martin TRTILEK and Jan HEJÁTKO. iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation. \textit{Frontiers in Plant Science}. Frontiers Media SA, 2023, vol.~14, April, p.~1-15. ISSN~1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2023.1093292.
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