J 2023

iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation

BALAKHONOVA, Veronika, Tereza DOBISOVÁ, Zuzana BENEDIKTY, Klara PANZAROVA, Jaromir PYTELA et. al.

Basic information

Original name

iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation

Authors

BALAKHONOVA, Veronika (643 Russian Federation, belonging to the institution), Tereza DOBISOVÁ (203 Czech Republic, belonging to the institution), Zuzana BENEDIKTY, Klara PANZAROVA, Jaromir PYTELA, Radka KOCI, Ioannis SPYROGLOU (300 Greece, belonging to the institution), Ingrid KOVÁČOVÁ (703 Slovakia, belonging to the institution), Dominique ARNAUD (250 France, belonging to the institution), Jan SKALÁK (203 Czech Republic, belonging to the institution), Martin TRTILEK and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)

Edition

Frontiers in Plant Science, Frontiers Media SA, 2023, 1664-462X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.600 in 2022

RIV identification code

RIV/00216224:14740/23:00132886

Organization unit

Central European Institute of Technology

UT WoS

000982095900001

Keywords in English

de-etiolation; chlorophyll biosynthesis; fluorescence; iReenCAM; cytokinins; ethylene; Arabidopsis

Tags

Tags

International impact, Reviewed
Změněno: 18/3/2024 14:50, Mgr. Marie Šípková, DiS.

Abstract

V originále

Seedling de-etiolation is one of the key stages of the plant life cycle, characterized by a strong rearrangement of the plant development and metabolism. The conversion of dark accumulated protochlorophyllide to chlorophyll in etioplasts of de-etiolating plants is taking place in order of ns to mu s after seedlings illumination, leading to detectable increase of chlorophyll levels in order of minutes after de-etiolation initiation. The highly complex chlorophyll biosynthesis integrates number of regulatory events including light and hormonal signaling, thus making de-etiolation an ideal model to study the underlying molecular mechanisms. Here we introduce the iReenCAM, a novel tool designed for non-invasive fluorescence-based quantitation of early stages of chlorophyll biosynthesis during de-etiolation with high spatial and temporal resolution. iReenCAM comprises customized HW configuration and optimized SW packages, allowing synchronized automated measurement and analysis of the acquired fluorescence image data. Using the system and carefully optimized protocol, we show tight correlation between the iReenCAM monitored fluorescence and HPLC measured chlorophyll accumulation during first 4h of seedling de-etiolation in wild type Arabidopsis and mutants with disturbed chlorophyll biosynthesis. Using the approach, we demonstrate negative effect of exogenously applied cytokinins and ethylene on chlorophyll biosynthesis during early de-etiolation. Accordingly, we identify type-B response regulators, the cytokinin-responsive transcriptional activators ARR1 and ARR12 as negative regulators of early chlorophyll biosynthesis, while contrasting response was observed in case of EIN2 and EIN3, the components of canonical ethylene signaling cascade. Knowing that, we propose the use of iReenCAM as a new phenotyping tool, suitable for quantitative and robust characterization of the highly dynamic response of seedling de-etiolation.

Links

EF16_026/0008446, research and development project
Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin