Dicer structure and function: conserved and evolving features

J 2023

Dicer structure and function: conserved and evolving features

ZAPLETAL, David, Karel KUBÍČEK, Petr SVOBODA and Richard ŠTEFL

Basic information

Original name

Dicer structure and function: conserved and evolving features

Authors

ZAPLETAL, David (203 Czech Republic, belonging to the institution), Karel KUBÍČEK (203 Czech Republic, belonging to the institution), Petr SVOBODA and Richard ŠTEFL (203 Czech Republic, guarantor, belonging to the institution)

Edition

EMBO reports, Wiley, 2023, 1469-221X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.700 in 2022

RIV identification code

RIV/00216224:14740/23:00132893

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.15252/embr.202357215

UT WoS

001005196500001

Keywords in English

Dicer; dsRBD; helicase; miRNA; siRNA

Abstract

V originále

RNase III Dicer produces small RNAs guiding sequence-specific regulations, with important biological roles in eukaryotes. Major Dicer-dependent mechanisms are RNA interference (RNAi) and microRNA (miRNA) pathways, which employ distinct types of small RNAs. Small interfering RNAs (siRNAs) for RNAi are produced by Dicer from long double-stranded RNA (dsRNA) as a pool of different small RNAs. In contrast, miRNAs have specific sequences because they are precisely cleaved out from small hairpin precursors. Some Dicer homologs efficiently generate both, siRNAs and miRNAs, while others are adapted for biogenesis of one small RNA type. Here, we review the wealth of recent structural analyses of animal and plant Dicers, which have revealed how different domains and their adaptations contribute to substrate recognition and cleavage in different organisms and pathways. These data imply that siRNA generation was Dicer's ancestral role and that miRNA biogenesis relies on derived features. While the key element of functional divergence is a RIG-I-like helicase domain, Dicer-mediated small RNA biogenesis also documents the impressive functional versatility of the dsRNA-binding domain.

Links

90140, large research infrastructures

Name: e-INFRA CZ

Citovat

ZAPLETAL, David, Karel KUBÍČEK, Petr SVOBODA and Richard ŠTEFL. Dicer structure and function: conserved and evolving features. EMBO reports. Wiley, 2023, vol. 24, No 7, p. 1-13. ISSN 1469-221X. Available from: https://dx.doi.org/10.15252/embr.202357215.

@article{2358654,
   author = {Zapletal, David and Kubíček, Karel and Svoboda, Petr and Štefl, Richard},
   article_number = {7},
   doi = {http://dx.doi.org/10.15252/embr.202357215},
   keywords = {Dicer; dsRBD; helicase; miRNA; siRNA},
   language = {eng},
   issn = {1469-221X},
   journal = {EMBO reports},
   title = {Dicer structure and function: conserved and evolving features},
   url = {https://www.embopress.org/doi/full/10.15252/embr.202357215},
   volume = {24},
   year = {2023}
}

TY  - JOUR
ID  - 2358654
AU  - Zapletal, David - Kubíček, Karel - Svoboda, Petr - Štefl, Richard
PY  - 2023
TI  - Dicer structure and function: conserved and evolving features
JF  - EMBO reports
VL  - 24
IS  - 7
SP  - 1-13
EP  - 1-13
PB  - Wiley
SN  - 1469221X
KW  - Dicer
KW  - dsRBD
KW  - helicase
KW  - miRNA
KW  - siRNA
UR  - https://www.embopress.org/doi/full/10.15252/embr.202357215
N2  - RNase III Dicer produces small RNAs guiding sequence-specific regulations, with important biological roles in eukaryotes. Major Dicer-dependent mechanisms are RNA interference (RNAi) and microRNA (miRNA) pathways, which employ distinct types of small RNAs. Small interfering RNAs (siRNAs) for RNAi are produced by Dicer from long double-stranded RNA (dsRNA) as a pool of different small RNAs. In contrast, miRNAs have specific sequences because they are precisely cleaved out from small hairpin precursors. Some Dicer homologs efficiently generate both, siRNAs and miRNAs, while others are adapted for biogenesis of one small RNA type. Here, we review the wealth of recent structural analyses of animal and plant Dicers, which have revealed how different domains and their adaptations contribute to substrate recognition and cleavage in different organisms and pathways. These data imply that siRNA generation was Dicer's ancestral role and that miRNA biogenesis relies on derived features. While the key element of functional divergence is a RIG-I-like helicase domain, Dicer-mediated small RNA biogenesis also documents the impressive functional versatility of the dsRNA-binding domain.
ER  -

ZAPLETAL, David, Karel KUBÍČEK, Petr SVOBODA and Richard ŠTEFL. Dicer structure and function: conserved and evolving features. \textit{EMBO reports}. Wiley, 2023, vol.~24, No~7, p.~1-13. ISSN~1469-221X. Available from: https://dx.doi.org/10.15252/embr.202357215.

Detailed Information on Publication Record