Detailed Information on Publication Record
2023
Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors
PÍREK, Pavlína, Karolína KRYŠTOFOVÁ, Ingrid KOVÁČOVÁ, Anna KROMEROVÁ, Dagmar ZACHOVÁ et. al.Basic information
Original name
Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors
Authors
PÍREK, Pavlína (203 Czech Republic, belonging to the institution), Karolína KRYŠTOFOVÁ (203 Czech Republic, belonging to the institution), Ingrid KOVÁČOVÁ (703 Slovakia, belonging to the institution), Anna KROMEROVÁ (203 Czech Republic, belonging to the institution), Dagmar ZACHOVÁ (203 Czech Republic, belonging to the institution), Ondřej HELIA (203 Czech Republic, belonging to the institution), Klara PANZAROVA (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Gabriela LOCHMANOVÁ (203 Czech Republic, belonging to the institution) and Miloslava FOJTOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
PLANTS-BASEL, BASEL, MDPI, 2023, 2223-7747
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.500 in 2022
RIV identification code
RIV/00216224:14740/23:00132929
Organization unit
Central European Institute of Technology
UT WoS
001133037600001
Keywords (in Czech)
Arabidopsis thaliana; tvorba mozolů; epigenetika; posttranslační modifikace histonů; hmotnostní spektrometrie; trichostatin A; sodium butyrate
Keywords in English
Arabidopsis thaliana; callus formation; epigenetics; histone posttranslational modifications; mass spectrometry; trichostatin A; sodium butyrate
Tags
International impact, Reviewed
Změněno: 8/4/2024 09:15, Mgr. Eva Dubská
Abstract
V originále
The ability for plant regeneration from dedifferentiated cells opens up the possibility for molecular bioengineering to produce crops with desirable traits. Developmental and environmental signals that control cell totipotency are regulated by gene expression via dynamic chromatin remodeling. Using a mass spectrometry-based approach, we investigated epigenetic changes to the histone proteins during callus formation from roots and shoots of Arabidopsis thaliana seedlings. Increased levels of the histone H3.3 variant were found to be the major and most prominent feature of 20-day calli, associated with chromatin relaxation. The methylation status in root- and shoot-derived calli reached the same level during long-term propagation, whereas differences in acetylation levels provided a long-lasting imprint of root and shoot origin. On the other hand, epigenetic signs of origin completely disappeared during 20 days of calli propagation in the presence of histone deacetylase inhibitors (HDACi), sodium butyrate, and trichostatin A. Each HDACi affected the state of post-translational histone modifications in a specific manner; NaB-treated calli were epigenetically more similar to root-derived calli, and TSA-treated calli resembled shoot-derived calli.
Links
| EF16_026/0008446, research and development project |
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| GA22-28190S, research and development project |
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| LM2023042, research and development project |
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| 90242, large research infrastructures |
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| 90254, large research infrastructures |
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