High temperature increases centromere-mediated genome
elimination frequency and enhances haploid induction in
Arabidopsis

J 2023

High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis

AHMADLI, Ulkar, Manikandan KALIDASS, Lucie CRHÁK KHAITOVÁ, Joerg FUCHS, Maria CUACOS et. al.

Basic information

Original name

High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis

Authors

AHMADLI, Ulkar, Manikandan KALIDASS, Lucie CRHÁK KHAITOVÁ (203 Czech Republic, belonging to the institution), Joerg FUCHS, Maria CUACOS, Dmitri DEMIDOV, Sheng ZUO (156 China, belonging to the institution), Jana PEČINKOVÁ (203 Czech Republic, belonging to the institution), Martin MASCHER, Mathieu INGOUFF, Stefan HECKMANN, Andreas HOUBEN, Karel ŘÍHA (203 Czech Republic, belonging to the institution) and Inna LERMONTOVA (guarantor)

Edition

Plant Communications, Elsevier, 2023, 2590-3462

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.500 in 2022

RIV identification code

RIV/00216224:14740/23:00132941

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.xplc.2022.100507

UT WoS

001007275100001

Keywords in English

centromere; kinetochore null 2; CENPC-k; cenh3-4; temperature stress; haploid induction

Abstract

V originále

Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggesting that haploid-inducing lines in crops can be identified in a naturally occurring or chemically induced mutant population, avoiding the generic modification (GM) approach at any stage. Furthermore, a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress, even though it did not induce haploids under standard conditions. Thus, we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.

Links

EF15_003/0000479, research and development project

Name: Regulace rostlinné meiózy

Citovat

AHMADLI, Ulkar, Manikandan KALIDASS, Lucie CRHÁK KHAITOVÁ, Joerg FUCHS, Maria CUACOS, Dmitri DEMIDOV, Sheng ZUO, Jana PEČINKOVÁ, Martin MASCHER, Mathieu INGOUFF, Stefan HECKMANN, Andreas HOUBEN, Karel ŘÍHA and Inna LERMONTOVA. High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis. Plant Communications. Elsevier, 2023, vol. 4, No 3, p. 1-13. ISSN 2590-3462. Available from: https://dx.doi.org/10.1016/j.xplc.2022.100507.

@article{2359499,
   author = {Ahmadli, Ulkar and Kalidass, Manikandan and Crhák Khaitová, Lucie and Fuchs, Joerg and Cuacos, Maria and Demidov, Dmitri and Zuo, Sheng and Pečinková, Jana and Mascher, Martin and Ingouff, Mathieu and Heckmann, Stefan and Houben, Andreas and Říha, Karel and Lermontova, Inna},
   article_number = {3},
   doi = {http://dx.doi.org/10.1016/j.xplc.2022.100507},
   keywords = {centromere; kinetochore null 2; CENPC-k; cenh3-4; temperature stress; haploid induction},
   language = {eng},
   issn = {2590-3462},
   journal = {Plant Communications},
   title = {High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis},
   url = {https://doi.org/10.1016/j.xplc.2022.100507},
   volume = {4},
   year = {2023}
}

TY  - JOUR
ID  - 2359499
AU  - Ahmadli, Ulkar - Kalidass, Manikandan - Crhák Khaitová, Lucie - Fuchs, Joerg - Cuacos, Maria - Demidov, Dmitri - Zuo, Sheng - Pečinková, Jana - Mascher, Martin - Ingouff, Mathieu - Heckmann, Stefan - Houben, Andreas - Říha, Karel - Lermontova, Inna
PY  - 2023
TI  - High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis
JF  - Plant Communications
VL  - 4
IS  - 3
SP  - 1-13
EP  - 1-13
PB  - Elsevier
SN  - 25903462
KW  - centromere
KW  - kinetochore null 2
KW  - CENPC-k
KW  - cenh3-4
KW  - temperature stress
KW  - haploid induction
UR  - https://doi.org/10.1016/j.xplc.2022.100507
N2  - Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggesting that haploid-inducing lines in crops can be identified in a naturally occurring or chemically induced mutant population, avoiding the generic modification (GM) approach at any stage. Furthermore, a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress, even though it did not induce haploids under standard conditions. Thus, we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.
ER  -

AHMADLI, Ulkar, Manikandan KALIDASS, Lucie CRHÁK KHAITOVÁ, Joerg FUCHS, Maria CUACOS, Dmitri DEMIDOV, Sheng ZUO, Jana PEČINKOVÁ, Martin MASCHER, Mathieu INGOUFF, Stefan HECKMANN, Andreas HOUBEN, Karel ŘÍHA and Inna LERMONTOVA. High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis. \textit{Plant Communications}. Elsevier, 2023, vol.~4, No~3, p.~1-13. ISSN~2590-3462. Available from: https://dx.doi.org/10.1016/j.xplc.2022.100507.

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