Detailed Information on Publication Record
2023
Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
HOFERKOVÁ, Eva, Soňa KADÁKOVÁ, Václav ŠEDA, Jan VERNER, Miroslav BOUDNÝ et. al.Basic information
Original name
Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
Authors
HOFERKOVÁ, Eva (203 Czech Republic, belonging to the institution), Soňa KADÁKOVÁ (703 Slovakia, belonging to the institution), Václav ŠEDA (203 Czech Republic, belonging to the institution), Jan VERNER (203 Czech Republic, belonging to the institution), Miroslav BOUDNÝ (203 Czech Republic, belonging to the institution), Gabriela MLADONICKÁ PAVLASOVÁ (203 Czech Republic, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Daniel FILIP (703 Slovakia, belonging to the institution), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Květoslava MATULOVÁ (203 Czech Republic), Hana SKUHROVÁ FRANCOVÁ (203 Czech Republic, belonging to the institution), Sonali SHARMA (356 India, belonging to the institution), Laura ONDRIŠOVÁ (703 Slovakia, belonging to the institution), Veronika PALUŠOVÁ (703 Slovakia, belonging to the institution), Lucy VOJTOVÁ (203 Czech Republic), Leoš KŘEN (203 Czech Republic), Michael DOUBEK (203 Czech Republic, belonging to the institution) and Marek MRÁZ (203 Czech Republic, guarantor, belonging to the institution)
Edition
CEITEC PhD Conference 2023, Brno, 2023
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30204 Oncology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14740/23:00132976
Organization unit
Central European Institute of Technology
Keywords in English
Chronic lymphocytic leukemia; CD40L; IL4; IL21; inhibitors; proliferation
Tags
Tags
Reviewed
Změněno: 30/3/2024 13:05, Mgr. Eva Dubská
Abstract
V originále
Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.
Links
| LX22NPO5102, research and development project |
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| MUNI/A/1330/2021, interní kód MU |
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| NU23-08-00448, research and development project |
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| 802644, interní kód MU |
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