Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.

HOFERKOVÁ, Eva, Soňa KADÁKOVÁ, Václav ŠEDA, Jan VERNER, Miroslav BOUDNÝ, Gabriela MLADONICKÁ PAVLASOVÁ, Tomáš LOJA, Daniel FILIP, Josef VEČEŘA, Květoslava MATULOVÁ, Hana SKUHROVÁ FRANCOVÁ, Sonali SHARMA, Laura ONDRIŠOVÁ, Veronika PALUŠOVÁ, Lucy VOJTOVÁ, Leoš KŘEN, Michael DOUBEK and Marek MRÁZ. Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation. In CEITEC PhD Conference 2023, Brno. 2023.

Basic information

• Original name: Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
• Authors: HOFERKOVÁ, Eva (203 Czech Republic, belonging to the institution), Soňa KADÁKOVÁ (703 Slovakia, belonging to the institution), Václav ŠEDA (203 Czech Republic, belonging to the institution), Jan VERNER (203 Czech Republic, belonging to the institution), Miroslav BOUDNÝ (203 Czech Republic, belonging to the institution), Gabriela MLADONICKÁ PAVLASOVÁ (203 Czech Republic, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Daniel FILIP (703 Slovakia, belonging to the institution), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Květoslava MATULOVÁ (203 Czech Republic), Hana SKUHROVÁ FRANCOVÁ (203 Czech Republic, belonging to the institution), Sonali SHARMA (356 India, belonging to the institution), Laura ONDRIŠOVÁ (703 Slovakia, belonging to the institution), Veronika PALUŠOVÁ (703 Slovakia, belonging to the institution), Lucy VOJTOVÁ (203 Czech Republic), Leoš KŘEN (203 Czech Republic), Michael DOUBEK (203 Czech Republic, belonging to the institution) and Marek MRÁZ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: CEITEC PhD Conference 2023, Brno, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30204 Oncology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/23:00132976
• Organization unit: Central European Institute of Technology
• Keywords in English: Chronic lymphocytic leukemia; CD40L; IL4; IL21; inhibitors; proliferation
• Tags: rivok
• Tags: Reviewed

Abstract

Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.

Links

• LX22NPO5102, research and development project: Name: Národní ústav pro výzkum rakoviny (Acronym: NÚVR)

Investor: Ministry of Education, Youth and Sports of the CR, National institute for cancer research, 5.1 EXCELES

• MUNI/A/1330/2021, interní kód MU: Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX (Acronym: VýDiTeHeMa IX)

Investor: Masaryk University

• NU23-08-00448, research and development project: Name: VÝVOJ MODELŮ PRO STUDIUM MIKROPROSTŘEDÍ A TERAPEUTICKÉ REZISTENCE U B BUNĚČNÝCH MALIGNIT

Investor: Ministry of Health of the CR, Development of models for the studies of microenvironment and therapeutic resistance in B cell malignancies, Subprogram 1 - standard

• 802644, interní kód MU: Name: Signaling propensity in the microenvironment of B cell chronic lymphocytic leukemia (Acronym: LeukemiaEnviron)

Investor: European Union, ERC (Excellent Science)