a 2023

Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.

HOFERKOVÁ, Eva, Soňa KADÁKOVÁ, Václav ŠEDA, Jan VERNER, Miroslav BOUDNÝ et. al.

Basic information

Original name

Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.

Authors

HOFERKOVÁ, Eva (203 Czech Republic, belonging to the institution), Soňa KADÁKOVÁ (703 Slovakia, belonging to the institution), Václav ŠEDA (203 Czech Republic, belonging to the institution), Jan VERNER (203 Czech Republic, belonging to the institution), Miroslav BOUDNÝ (203 Czech Republic, belonging to the institution), Gabriela MLADONICKÁ PAVLASOVÁ (203 Czech Republic, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Daniel FILIP (703 Slovakia, belonging to the institution), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Květoslava MATULOVÁ (203 Czech Republic), Hana SKUHROVÁ FRANCOVÁ (203 Czech Republic, belonging to the institution), Sonali SHARMA (356 India, belonging to the institution), Laura ONDRIŠOVÁ (703 Slovakia, belonging to the institution), Veronika PALUŠOVÁ (703 Slovakia, belonging to the institution), Lucy VOJTOVÁ (203 Czech Republic), Leoš KŘEN (203 Czech Republic), Michael DOUBEK (203 Czech Republic, belonging to the institution) and Marek MRÁZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

CEITEC PhD Conference 2023, Brno, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30204 Oncology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14740/23:00132976

Organization unit

Central European Institute of Technology

Keywords in English

Chronic lymphocytic leukemia; CD40L; IL4; IL21; inhibitors; proliferation

Tags

Tags

Reviewed
Změněno: 30/3/2024 13:05, Mgr. Eva Dubská

Abstract

V originále

Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.

Links

LX22NPO5102, research and development project
Name: Národní ústav pro výzkum rakoviny (Acronym: NÚVR)
Investor: Ministry of Education, Youth and Sports of the CR, National institute for cancer research, 5.1 EXCELES
MUNI/A/1330/2021, interní kód MU
Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX (Acronym: VýDiTeHeMa IX)
Investor: Masaryk University
NU23-08-00448, research and development project
Name: VÝVOJ MODELŮ PRO STUDIUM MIKROPROSTŘEDÍ A TERAPEUTICKÉ REZISTENCE U B BUNĚČNÝCH MALIGNIT
Investor: Ministry of Health of the CR, Development of models for the studies of microenvironment and therapeutic resistance in B cell malignancies, Subprogram 1 - standard
802644, interní kód MU
Name: Signaling propensity in the microenvironment of B cell chronic lymphocytic leukemia (Acronym: LeukemiaEnviron)
Investor: European Union, ERC (Excellent Science)