Changes in gene expression in pressure ulcers debrided by
different approaches - a pilot study

J 2023

Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study

SAMADIAN, Amir, Monika KRATOCHVÍLOVÁ, Alica HOKYNKOVÁ, Petr ŠÍN, Marie NOVÁKOVÁ et. al.

Basic information

Original name

Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study

Authors

SAMADIAN, Amir (364 Islamic Republic of Iran, belonging to the institution), Monika KRATOCHVÍLOVÁ (203 Czech Republic, belonging to the institution), Alica HOKYNKOVÁ (703 Slovakia, belonging to the institution), Petr ŠÍN (203 Czech Republic, belonging to the institution), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Petr ŠTĚPKA (203 Czech Republic, belonging to the institution), Andrea POKORNÁ (203 Czech Republic, belonging to the institution) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Physiological research, Praha, Institute of Physiology of the Czechoslovak Academy of Sciences, 2023, 0862-8408

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.100 in 2022

RIV identification code

RIV/00216224:14110/23:00134692

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.33549/physiolres.935222

UT WoS

001198072000009

Keywords in English

Pressure ulcers; Debridement; Wound healing; Oxidative stress; Gene expression

Abstract

V originále

Pressure ulcers (PUs), also known as pressure injuries, are chronic wounds that represent potential lifelong complications. Pressure ulcers of a deep category (III and IV) are often indicated for surgical treatment - debridement and surgical reconstruction. Sharp surgical debridement is widely used in the debridement of PUs; however, the Versajet® hydrosurgery system is becoming an increasingly popular tool for tangential excision in surgery due to its numerous advantages. This work focused on the expression of selected genes, especially those associated with oxidative stress, in PUs debrided by two approaches - sharp surgical debridement and debridement using Versajet® hydrosurgery system. Expression of following genes was evaluated: NFE2L2, ACTA2, NFKB1, VEGFA, MKI67, HMOX1, HMOX2, HIF1A, and SOD2. ACTB and PSMB were used as housekeeping genes. So far, five patients have been enrolled in the study. Preliminary results suggest no significant difference in gene expression with different pressure ulcer treatment approaches except NFE2L2, despite the macroscopic differences. However, the results revealed correlations between the expression of some genes, namely HIF1A and SOD2, VEGFA and SOD2 and VEGFA and HIF1A. These results may indicate a connection between hypoxia, oxidative stress, pressure ulcer healing processes and angiogenesis.

Links

NU21-09-00541, research and development project

Name: Role oxidativního stresu při hojení dekubitů u pacientů s míšní lézí

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

SAMADIAN, Amir, Monika KRATOCHVÍLOVÁ, Alica HOKYNKOVÁ, Petr ŠÍN, Marie NOVÁKOVÁ, Petr ŠTĚPKA, Andrea POKORNÁ and Petr BABULA. Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study. Physiological research. Praha: Institute of Physiology of the Czechoslovak Academy of Sciences, 2023, vol. 72, Supplementum 5, p. "S535"-"S542", 8 pp. ISSN 0862-8408. Available from: https://dx.doi.org/10.33549/physiolres.935222.

@article{2360027,
   author = {Samadian, Amir and Kratochvílová, Monika and Hokynková, Alica and Šín, Petr and Nováková, Marie and Štěpka, Petr and Pokorná, Andrea and Babula, Petr},
   article_location = {Praha},
   article_number = {Supplementum 5},
   doi = {http://dx.doi.org/10.33549/physiolres.935222},
   keywords = {Pressure ulcers; Debridement; Wound healing; Oxidative stress; Gene expression},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological research},
   title = {Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study},
   url = {https://www.biomed.cas.cz/physiolres/pdf/72/72_S535.pdf},
   volume = {72},
   year = {2023}
}

TY  - JOUR
ID  - 2360027
AU  - Samadian, Amir - Kratochvílová, Monika - Hokynková, Alica - Šín, Petr - Nováková, Marie - Štěpka, Petr - Pokorná, Andrea - Babula, Petr
PY  - 2023
TI  - Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study
JF  - Physiological research
VL  - 72
IS  - Supplementum 5
SP  - "S535"-"S542"
EP  - "S535"-"S542"
PB  - Institute of Physiology of the Czechoslovak Academy of Sciences
SN  - 08628408
KW  - Pressure ulcers
KW  - Debridement
KW  - Wound healing
KW  - Oxidative stress
KW  - Gene expression
UR  - https://www.biomed.cas.cz/physiolres/pdf/72/72_S535.pdf
N2  - Pressure ulcers (PUs), also known as pressure injuries, are chronic wounds that represent potential lifelong complications. Pressure ulcers of a deep category (III and IV) are often indicated for surgical treatment - debridement and surgical reconstruction. Sharp surgical debridement is widely used in the debridement of PUs; however, the Versajet® hydrosurgery system is becoming an increasingly popular tool for tangential excision in surgery due to its numerous advantages. This work focused on the expression of selected genes, especially those associated with oxidative stress, in PUs debrided by two approaches - sharp surgical debridement and debridement using Versajet® hydrosurgery system. Expression of following genes was evaluated: NFE2L2, ACTA2, NFKB1, VEGFA, MKI67, HMOX1, HMOX2, HIF1A, and SOD2. ACTB and PSMB were used as housekeeping genes. So far, five patients have been enrolled in the study. Preliminary results suggest no significant difference in gene expression with different pressure ulcer treatment approaches except NFE2L2, despite the macroscopic differences. However, the results revealed correlations between the expression of some genes, namely HIF1A and SOD2, VEGFA and SOD2 and VEGFA and HIF1A. These results may indicate a connection between hypoxia, oxidative stress, pressure ulcer healing processes and angiogenesis.
ER  -

SAMADIAN, Amir, Monika KRATOCHVÍLOVÁ, Alica HOKYNKOVÁ, Petr ŠÍN, Marie NOVÁKOVÁ, Petr ŠTĚPKA, Andrea POKORNÁ and Petr BABULA. Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study. \textit{Physiological research}. Praha: Institute of Physiology of the Czechoslovak Academy of Sciences, 2023, vol.~72, Supplementum 5, p.~''S535''-''S542'', 8 pp. ISSN~0862-8408. Available from: https://dx.doi.org/10.33549/physiolres.935222.

Detailed Information on Publication Record