Circulation of Anaplasma phagocytophilum among invasive and
native carnivore species living in sympatry in Poland

J 2023

Circulation of Anaplasma phagocytophilum among invasive and native carnivore species living in sympatry in Poland

LESICZKA, Paulina Maria, Izabella MYSLIWY, Katarzyna BUNKOWSKA-GAWLIK, David MODRÝ, Kristyna HRAZDILOVA et. al.

Basic information

Original name

Circulation of Anaplasma phagocytophilum among invasive and native carnivore species living in sympatry in Poland

Authors

LESICZKA, Paulina Maria (guarantor), Izabella MYSLIWY, Katarzyna BUNKOWSKA-GAWLIK, David MODRÝ (203 Czech Republic, belonging to the institution), Kristyna HRAZDILOVA, Joanna HILDEBRAND and Agnieszka PEREC-MATYSIAK

Edition

PARASITES & VECTORS, LONDON, BIOMED CENTRAL LTD, 2023, 1756-3305

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30310 Parasitology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.200 in 2022

RIV identification code

RIV/00216224:14310/23:00133007

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/s13071-023-05996-7

UT WoS

001087701400001

Keywords in English

Anaplasma phagocytophilum; Carnivores; Meles meles; Martes spp.; Nyctereutes procyonides; Procyon lotor; Vulpes vulpes; Invasive species

Abstract

V originále

Background Anaplasma phagocytophilum is characterized by a worldwide distribution and distinguished from other Anaplasmataceae by the broadest range of mammalian hosts and high genetic diversity. The role carnivores play in the life cycle of A. phagocytophilum in Europe is uncertain. Currently, only the red fox is considered a suitable reservoir host. In this study, we focused on native and invasive medium-sized carnivore species that live in sympatry and represent the most abundant species of wild carnivores in Poland.Methods A total of 275 individual spleen samples from six carnivore species (Vulpes vulpes, Meles meles, Procyon lotor, Nyctereutes procyonoides and Martes spp.) were screened combining nested PCR and sequencing for A. phagocytophilum targeting a partial groEL gene with subsequent phylogenetic analysis inferred by the maximum likelihood method.Results The DNA of A. phagocytophilum was detected in 16 of 275 individuals (5.8%). Eight unique genetic variants of A. phagocytophilum were obtained. All detected haplotypes clustered in the clade representing European ecotype I. Three variants belonged to the subclade with European human cases together with strains from dogs, foxes, cats, and wild boars.Conclusions While carnivores might have a restricted role in the dissemination of A. phagocytophilum due to their relatively low to moderate infection rates, they hold significance as hosts for ticks. Consequently, they could contribute to the transmission of tick-borne infections to humans indirectly, primarily through tick infection. This underscores the potential risk of urbanization for the A. phagocytophilum life cycle, further emphasizing the need for comprehensive understanding of its ecological dynamics.

Citovat

LESICZKA, Paulina Maria, Izabella MYSLIWY, Katarzyna BUNKOWSKA-GAWLIK, David MODRÝ, Kristyna HRAZDILOVA, Joanna HILDEBRAND and Agnieszka PEREC-MATYSIAK. Circulation of Anaplasma phagocytophilum among invasive and native carnivore species living in sympatry in Poland. PARASITES & VECTORS. LONDON: BIOMED CENTRAL LTD, 2023, vol. 16, No 1, p. 1-7. ISSN 1756-3305. Available from: https://dx.doi.org/10.1186/s13071-023-05996-7.

@article{2360138,
   author = {Lesiczka, Paulina Maria and Mysliwy, Izabella and BunkowskaandGawlik, Katarzyna and Modrý, David and Hrazdilova, Kristyna and Hildebrand, Joanna and PerecandMatysiak, Agnieszka},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s13071-023-05996-7},
   keywords = {Anaplasma phagocytophilum; Carnivores; Meles meles; Martes spp.; Nyctereutes procyonides; Procyon lotor; Vulpes vulpes; Invasive species},
   language = {eng},
   issn = {1756-3305},
   journal = {PARASITES & VECTORS},
   title = {Circulation of Anaplasma phagocytophilum among invasive and native carnivore species living in sympatry in Poland},
   url = {https://doi.org/10.1186/s13071-023-05996-7},
   volume = {16},
   year = {2023}
}

TY  - JOUR
ID  - 2360138
AU  - Lesiczka, Paulina Maria - Mysliwy, Izabella - Bunkowska-Gawlik, Katarzyna - Modrý, David - Hrazdilova, Kristyna - Hildebrand, Joanna - Perec-Matysiak, Agnieszka
PY  - 2023
TI  - Circulation of Anaplasma phagocytophilum among invasive and native carnivore species living in sympatry in Poland
JF  - PARASITES & VECTORS
VL  - 16
IS  - 1
SP  - 1-7
EP  - 1-7
PB  - BIOMED CENTRAL LTD
SN  - 17563305
KW  - Anaplasma phagocytophilum
KW  - Carnivores
KW  - Meles meles
KW  - Martes spp.
KW  - Nyctereutes procyonides
KW  - Procyon lotor
KW  - Vulpes vulpes
KW  - Invasive species
UR  - https://doi.org/10.1186/s13071-023-05996-7
N2  - Background Anaplasma phagocytophilum is characterized by a worldwide distribution and distinguished from other Anaplasmataceae by the broadest range of mammalian hosts and high genetic diversity. The role carnivores play in the life cycle of A. phagocytophilum in Europe is uncertain. Currently, only the red fox is considered a suitable reservoir host. In this study, we focused on native and invasive medium-sized carnivore species that live in sympatry and represent the most abundant species of wild carnivores in Poland.Methods A total of 275 individual spleen samples from six carnivore species (Vulpes vulpes, Meles meles, Procyon lotor, Nyctereutes procyonoides and Martes spp.) were screened combining nested PCR and sequencing for A. phagocytophilum targeting a partial groEL gene with subsequent phylogenetic analysis inferred by the maximum likelihood method.Results The DNA of A. phagocytophilum was detected in 16 of 275 individuals (5.8%). Eight unique genetic variants of A. phagocytophilum were obtained. All detected haplotypes clustered in the clade representing European ecotype I. Three variants belonged to the subclade with European human cases together with strains from dogs, foxes, cats, and wild boars.Conclusions While carnivores might have a restricted role in the dissemination of A. phagocytophilum due to their relatively low to moderate infection rates, they hold significance as hosts for ticks. Consequently, they could contribute to the transmission of tick-borne infections to humans indirectly, primarily through tick infection. This underscores the potential risk of urbanization for the A. phagocytophilum life cycle, further emphasizing the need for comprehensive understanding of its ecological dynamics.
ER  -

LESICZKA, Paulina Maria, Izabella MYSLIWY, Katarzyna BUNKOWSKA-GAWLIK, David MODRÝ, Kristyna HRAZDILOVA, Joanna HILDEBRAND and Agnieszka PEREC-MATYSIAK. Circulation of Anaplasma phagocytophilum among invasive and native carnivore species living in sympatry in Poland. \textit{PARASITES \&{}amp; VECTORS}. LONDON: BIOMED CENTRAL LTD, 2023, vol.~16, No~1, p.~1-7. ISSN~1756-3305. Available from: https://dx.doi.org/10.1186/s13071-023-05996-7.

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