Revealing the complexity of mesenchymal stem cell niche of
continuously growing teeth

a 2022

Revealing the complexity of mesenchymal stem cell niche of continuously growing teeth

KŘIVÁNEK, Jan, Josef LAVICKÝ, Adam BOGDANOVIČ, Marcos GONZÁLEZ LÓPEZ, Vladislav RAKULTSEV et. al.

Basic information

Original name

Revealing the complexity of mesenchymal stem cell niche of continuously growing teeth

Authors

KŘIVÁNEK, Jan, Josef LAVICKÝ, Adam BOGDANOVIČ, Marcos GONZÁLEZ LÓPEZ and Vladislav RAKULTSEV

Edition

14th Tooth Morphogenesis & Differentiation Meeting, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10605 Developmental biology

Confidentiality degree

není předmětem státního či obchodního tajemství

Abstract

V originále

The continuous growth of mouse incisor is fully dependent on the activity of epithelial and mesenchymal stem cell niches positioned in the apical area of the tooth. Unlike the dental epithelium, in which the stem cell niche is spatially limited, the situation in the dental mesenchyme is far more complicated. Although several distinct types and sources of dental mesenchymal stem cells have been described, the general consensus about their dynamics and mechanisms regulating their activity has still not been resolved. Here we present a new population of rare quiescent mesenchymal stem cells in teeth. These unprecedently spatially restricted mesenchymal stem cells give rise to different mesenchymal cell types in teeth, including pulp cells and odontoblasts. Using scRNA-seq and lineage tracing we have uncovered their differentiation potential in the healthy and damaged teeth. Moreover, we show on the cellular and molecular levels, how this unique population of stem cells is able to react to the induced injury. Finally, we also suggest that they have a wider differentiation potential than expected. Taken together, this work provides new insight into the level of complexity of the dental mesenchyme and demonstrates new ways in which continuously growing teeth can respond to a changing outer environment.

Links

GA22-02794S, research and development project

Name: Mechanorecepce jako mechanizmus řídící odontogenezi napříč obratlovci

Investor: Czech Science Foundation

Citovat

KŘIVÁNEK, Jan, Josef LAVICKÝ, Adam BOGDANOVIČ, Marcos GONZÁLEZ LÓPEZ and Vladislav RAKULTSEV. Revealing the complexity of mesenchymal stem cell niche of continuously growing teeth. In 14th Tooth Morphogenesis & Differentiation Meeting. 2022.

@proceedings{2360320,
   author = {Křivánek, Jan and Lavický, Josef and Bogdanovič, Adam and González López, Marcos and Rakultsev, Vladislav},
   booktitle = {14th Tooth Morphogenesis & Differentiation Meeting},
   language = {eng},
   title = {Revealing the complexity of mesenchymal stem cell niche of continuously growing teeth},
   year = {2022}
}

TY  - CONF
ID  - 2360320
AU  - Křivánek, Jan - Lavický, Josef - Bogdanovič, Adam - González López, Marcos - Rakultsev, Vladislav
PY  - 2022
TI  - Revealing the complexity of mesenchymal stem cell niche of continuously growing teeth
N2  - The continuous growth of mouse incisor is fully dependent on the activity of epithelial and mesenchymal stem cell niches positioned in the apical area of the tooth. Unlike the dental epithelium, in which the stem cell niche is spatially limited, the situation in the dental mesenchyme is far more complicated. Although several distinct types and sources of dental mesenchymal stem cells have been described, the general consensus about their dynamics and mechanisms regulating their activity has still not been resolved. Here we present a new population of rare quiescent mesenchymal stem cells in teeth. These unprecedently spatially restricted mesenchymal stem cells give rise to different mesenchymal cell types in teeth, including pulp cells and odontoblasts. Using scRNA-seq and lineage tracing we have uncovered their differentiation potential in the healthy and damaged teeth. Moreover, we show on the cellular and molecular levels, how this unique population of stem cells is able to react to the induced injury. Finally, we also suggest that they have a wider differentiation potential than expected. Taken together, this work provides new insight into the level of complexity of the dental mesenchyme and demonstrates new ways in which continuously growing teeth can respond to a changing outer environment.
ER  -

KŘIVÁNEK, Jan, Josef LAVICKÝ, Adam BOGDANOVIČ, Marcos GONZÁLEZ LÓPEZ and Vladislav RAKULTSEV. Revealing the complexity of mesenchymal stem cell niche of continuously growing teeth. In \textit{14th Tooth Morphogenesis \&{} Differentiation Meeting}. 2022.

Detailed Information on Publication Record