Cryoresistance of Antarctic endemic lichen Himantormia
lugubris: Analysis of photosystem II functionality using a
constant-rate cooling approach

J 2023

Cryoresistance of Antarctic endemic lichen Himantormia lugubris: Analysis of photosystem II functionality using a constant-rate cooling approach

HÁJEK, Josef, Angelica CASANOVA-KATNY, Miloš BARTÁK and Jiří SEKERÁK

Basic information

Original name

Cryoresistance of Antarctic endemic lichen Himantormia lugubris: Analysis of photosystem II functionality using a constant-rate cooling approach

Authors

HÁJEK, Josef (203 Czech Republic, guarantor, belonging to the institution), Angelica CASANOVA-KATNY, Miloš BARTÁK (203 Czech Republic, belonging to the institution) and Jiří SEKERÁK (203 Czech Republic, belonging to the institution)

Edition

Czech Polar Reports, Brno, Masaryk University, 2023, 1805-0689

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.000 in 2022

RIV identification code

RIV/00216224:14310/23:00133088

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.5817/CPR2023-1-10

UT WoS

001098584100001

Keywords in English

freezing; photosynthetic apparatus; cooling point; King George Island; differential method

Abstract

V originále

It is well established that lichens from polar regions of the Earth are capable to perform photosynthesis at sub-zero temperatures. Majority of them show a high degree of cryoresistance, however, species-specific differences exist. Therefore, the aim of our study was to evaluate behaviour of primary photochemical processes of photosynthesis in Antarctic endemic species Himantormia lugubris at sub-zero temperature. For the purpose, the method of constant rate (2 degrees C min-1) cooling (from +20 to-40 degrees C) with simultaneous measurements of chlorophyll fluorescence parameters related to photosystem II (PSII) was used. During the cooling, potential yield of photosynthetic processes in PSII (FV/FM), and effective quantum yield of PSII (phi PSII) were measured in 30 s interval. From the FV/FM and phi PSII data sets, S-curves reflecting temperature dependence of the two chlorophyll fluorescence parameters were constructed and analyzed. The S-curves were found tri-phasic in response to sample temperature decline: (1) slight or no decline phase, (2) rapid decline phase, followed by (3) slow change reaching critical temperature at which the primary photosynthetic processes were fully inhibited. Critical temperature was found-30 and-20 degrees C for FV/FM, and phi PSII, respectively. The latter critical temperature was accompanied by an increase in background chlorophyll fluorescence (F0) indicating inhibition of energy transfer from light-harvesting complexes to core of PSII. A newly-designed chlorophyll fluorescence parameter (a differential, i.e. the difference between the maximum value-normalized FV/FM, and phi PSII) was used in order to evaluate the temperature at which the processes related to photosynthetic electron flow through thylakoid membrane carriers (phi PSII) and the energy flow through PSII (FV/FM) differed to a largest extent. This parameters proved to be temperature-dependent and useful in the evaluation of cryoresistance. Based on our study, H. lugubris, its primary photosynthetic processes in particular, might be considered as higly resistant to sub-zero temperature.

Links

EF16_013/0001708, research and development project

Name: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)

VAN 2023, interní kód MU

Name: Český antarktický výzkumný program 2023

Investor: Ministry of Education, Youth and Sports of the CR, Czech Antarctic Research Programme 2023, Antarctic research

Citovat

HÁJEK, Josef, Angelica CASANOVA-KATNY, Miloš BARTÁK and Jiří SEKERÁK. Cryoresistance of Antarctic endemic lichen Himantormia lugubris: Analysis of photosystem II functionality using a constant-rate cooling approach. Czech Polar Reports. Brno: Masaryk University, 2023, vol. 13, No 1, p. 112-120. ISSN 1805-0689. Available from: https://dx.doi.org/10.5817/CPR2023-1-10.

@article{2362563,
   author = {Hájek, Josef and CasanovaandKatny, Angelica and Barták, Miloš and Sekerák, Jiří},
   article_location = {Brno},
   article_number = {1},
   doi = {http://dx.doi.org/10.5817/CPR2023-1-10},
   keywords = {freezing; photosynthetic apparatus; cooling point; King George Island; differential method},
   language = {eng},
   issn = {1805-0689},
   journal = {Czech Polar Reports},
   title = {Cryoresistance of Antarctic endemic lichen Himantormia lugubris: Analysis of photosystem II functionality using a constant-rate cooling approach},
   url = {https://journals.muni.cz/CPR/article/view/37053},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2362563
AU  - Hájek, Josef - Casanova-Katny, Angelica - Barták, Miloš - Sekerák, Jiří
PY  - 2023
TI  - Cryoresistance of Antarctic endemic lichen Himantormia lugubris: Analysis of photosystem II functionality using a constant-rate cooling approach
JF  - Czech Polar Reports
VL  - 13
IS  - 1
SP  - 112-120
EP  - 112-120
PB  - Masaryk University
SN  - 18050689
KW  - freezing
KW  - photosynthetic apparatus
KW  - cooling point
KW  - King George Island
KW  - differential method
UR  - https://journals.muni.cz/CPR/article/view/37053
N2  - It is well established that lichens from polar regions of the Earth are capable to perform photosynthesis at sub-zero temperatures. Majority of them show a high degree of cryoresistance, however, species-specific differences exist. Therefore, the aim of our study was to evaluate behaviour of primary photochemical processes of photosynthesis in Antarctic endemic species Himantormia lugubris at sub-zero temperature. For the purpose, the method of constant rate (2 degrees C min-1) cooling (from +20 to-40 degrees C) with simultaneous measurements of chlorophyll fluorescence parameters related to photosystem II (PSII) was used. During the cooling, potential yield of photosynthetic processes in PSII (FV/FM), and effective quantum yield of PSII (phi PSII) were measured in 30 s interval. From the FV/FM and phi PSII data sets, S-curves reflecting temperature dependence of the two chlorophyll fluorescence parameters were constructed and analyzed. The S-curves were found tri-phasic in response to sample temperature decline: (1) slight or no decline phase, (2) rapid decline phase, followed by (3) slow change reaching critical temperature at which the primary photosynthetic processes were fully inhibited. Critical temperature was found-30 and-20 degrees C for FV/FM, and phi PSII, respectively. The latter critical temperature was accompanied by an increase in background chlorophyll fluorescence (F0) indicating inhibition of energy transfer from light-harvesting complexes to core of PSII. A newly-designed chlorophyll fluorescence parameter (a differential, i.e. the difference between the maximum value-normalized FV/FM, and phi PSII) was used in order to evaluate the temperature at which the processes related to photosynthetic electron flow through thylakoid membrane carriers (phi PSII) and the energy flow through PSII (FV/FM) differed to a largest extent. This parameters proved to be temperature-dependent and useful in the evaluation of cryoresistance. Based on our study, H. lugubris, its primary photosynthetic processes in particular, might be considered as higly resistant to sub-zero temperature.
ER  -

HÁJEK, Josef, Angelica CASANOVA-KATNY, Miloš BARTÁK and Jiří SEKERÁK. Cryoresistance of Antarctic endemic lichen Himantormia lugubris: Analysis of photosystem II functionality using a constant-rate cooling approach. \textit{Czech Polar Reports}. Brno: Masaryk University, 2023, vol.~13, No~1, p.~112-120. ISSN~1805-0689. Available from: https://dx.doi.org/10.5817/CPR2023-1-10.

Detailed Information on Publication Record