Formulation optimization and evaluation of oromucosal in situ
gel loaded with silver nanoparticles prepared by green
biosynthesis

J 2024

Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis

ROHAĽOVÁ, Simona, Tomas WOLASCHKA, Ludmila BALAZOVA, Katarina PAULOVICOVA, Jana TOTHOVA et. al.

Basic information

Original name

Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis

Authors

ROHAĽOVÁ, Simona (703 Slovakia, belonging to the institution), Tomas WOLASCHKA, Ludmila BALAZOVA, Katarina PAULOVICOVA, Jana TOTHOVA, Sylvie PAVLOKOVÁ (203 Czech Republic, belonging to the institution), Martin STAHORSKY and Jan GAJDZIOK (203 Czech Republic, guarantor, belonging to the institution)

Edition

European Journal of Pharmaceutical Sciences, Amsterdam, Elsevier, 2024, 0928-0987

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1016/j.ejps.2023.106683

UT WoS

001155785100001

Keywords in English

In situ gel; Oral disease; Silver nanoparticles; Agrimonia eupatoria L.; Smart polymer; Principal component analysis

Abstract

V originále

Treating oral diseases remains challenging as API is quickly washed out of the application site by saliva turnover and mouth movements. In situ gels are a class of application forms that present sol-gel transition's ability as a response to stimuli. Their tunable properties are provided using smart polymers responsible for stimuli sensitivity, often providing mucoadhesivity. In this study, antimicrobial in situ gels of thermosensitive and pHsensitive polymers loaded with silver nanoparticles were prepared and evaluated. The nanoparticles were prepared by green synthesis using Agrimonia eupatoria L. extract. According to the data analysis, the in situ gel with the most promising profile contained 15 % of Pluronic (R) F-127, 0.25 % of methylcellulose, and 0.1 % of Noveon (R) AA-1. Pluronic (R) F-127 and methylcellulose significantly increased the viscosity of in situ gels at 37 degree celsius and shear rates similar to speaking and swallowing. At 20 degree celsius, a behavior close to a Newtonian fluid was observed while being easily injectable (injection force 13.455 +/- 1.973 N). The viscosity of the formulation increased with temperature and reached 2962.77 +/- 63.37 mPa center dot s (37 degree celsius). A temperature increase led to increased adhesiveness and rigidity of the formulation. The critical sol-gel transition temperature at physiological pH was 32.65 +/- 0.35 degree celsius. 96.77 +/- 3.26 % of Ag NPs were released by erosion and dissolution of the gel after 40 min. The determination of MIC showed effect against E. coli and S. aureus (0.0625 mM and 0.5000 mM, respectively). The relative inhibition zone diameter of the in situ gel was 73.32 +/- 11.06 % compared to gentamicin sulfate. This work discusses the optimization of the formulation of novel antibacterial in situ gel for oromucosal delivery, analyses the impact of the concentration of excipients on the dependent variables, and suggests appropriate evaluation of the formulation in terms of its indication. This study offers a promising dosage form for local treatment of oral diseases.

Citovat

ROHAĽOVÁ, Simona, Tomas WOLASCHKA, Ludmila BALAZOVA, Katarina PAULOVICOVA, Jana TOTHOVA, Sylvie PAVLOKOVÁ, Martin STAHORSKY and Jan GAJDZIOK. Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis. European Journal of Pharmaceutical Sciences. Amsterdam: Elsevier, 2024, vol. 193, February, p. 1-15. ISSN 0928-0987. Available from: https://dx.doi.org/10.1016/j.ejps.2023.106683.

@article{2362898,
   author = {Rohaľová, Simona and Wolaschka, Tomas and Balazova, Ludmila and Paulovicova, Katarina and Tothova, Jana and Pavloková, Sylvie and Stahorsky, Martin and Gajdziok, Jan},
   article_location = {Amsterdam},
   article_number = {February},
   doi = {http://dx.doi.org/10.1016/j.ejps.2023.106683},
   keywords = {In situ gel; Oral disease; Silver nanoparticles; Agrimonia eupatoria L.; Smart polymer; Principal component analysis},
   language = {eng},
   issn = {0928-0987},
   journal = {European Journal of Pharmaceutical Sciences},
   title = {Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis},
   url = {https://www.sciencedirect.com/science/article/pii/S0928098723003111?via%3Dihub},
   volume = {193},
   year = {2024}
}

TY  - JOUR
ID  - 2362898
AU  - Rohaľová, Simona - Wolaschka, Tomas - Balazova, Ludmila - Paulovicova, Katarina - Tothova, Jana - Pavloková, Sylvie - Stahorsky, Martin - Gajdziok, Jan
PY  - 2024
TI  - Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis
JF  - European Journal of Pharmaceutical Sciences
VL  - 193
IS  - February
SP  - 1-15
EP  - 1-15
PB  - Elsevier
SN  - 09280987
KW  - In situ gel
KW  - Oral disease
KW  - Silver nanoparticles
KW  - Agrimonia eupatoria L.
KW  - Smart polymer
KW  - Principal component analysis
UR  - https://www.sciencedirect.com/science/article/pii/S0928098723003111?via%3Dihub
N2  - Treating oral diseases remains challenging as API is quickly washed out of the application site by saliva turnover and mouth movements. In situ gels are a class of application forms that present sol-gel transition's ability as a response to stimuli. Their tunable properties are provided using smart polymers responsible for stimuli sensitivity, often providing mucoadhesivity. In this study, antimicrobial in situ gels of thermosensitive and pHsensitive polymers loaded with silver nanoparticles were prepared and evaluated. The nanoparticles were prepared by green synthesis using Agrimonia eupatoria L. extract. According to the data analysis, the in situ gel with the most promising profile contained 15 % of Pluronic (R) F-127, 0.25 % of methylcellulose, and 0.1 % of Noveon (R) AA-1. Pluronic (R) F-127 and methylcellulose significantly increased the viscosity of in situ gels at 37 degree celsius and shear rates similar to speaking and swallowing. At 20 degree celsius, a behavior close to a Newtonian fluid was observed while being easily injectable (injection force 13.455 +/- 1.973 N). The viscosity of the formulation increased with temperature and reached 2962.77 +/- 63.37 mPa center dot s (37 degree celsius). A temperature increase led to increased adhesiveness and rigidity of the formulation. The critical sol-gel transition temperature at physiological pH was 32.65 +/- 0.35 degree celsius. 96.77 +/- 3.26 % of Ag NPs were released by erosion and dissolution of the gel after 40 min. The determination of MIC showed effect against E. coli and S. aureus (0.0625 mM and 0.5000 mM, respectively). The relative inhibition zone diameter of the in situ gel was 73.32 +/- 11.06 % compared to gentamicin sulfate. This work discusses the optimization of the formulation of novel antibacterial in situ gel for oromucosal delivery, analyses the impact of the concentration of excipients on the dependent variables, and suggests appropriate evaluation of the formulation in terms of its indication. This study offers a promising dosage form for local treatment of oral diseases.
ER  -

ROHAĽOVÁ, Simona, Tomas WOLASCHKA, Ludmila BALAZOVA, Katarina PAULOVICOVA, Jana TOTHOVA, Sylvie PAVLOKOVÁ, Martin STAHORSKY and Jan GAJDZIOK. Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis. \textit{European Journal of Pharmaceutical Sciences}. Amsterdam: Elsevier, 2024, vol.~193, February, p.~1-15. ISSN~0928-0987. Available from: https://dx.doi.org/10.1016/j.ejps.2023.106683.

Detailed Information on Publication Record