ROHAĽOVÁ, Simona, Tomas WOLASCHKA, Ludmila BALAZOVA, Katarina PAULOVICOVA, Jana TOTHOVA, Sylvie PAVLOKOVÁ, Martin STAHORSKY and Jan GAJDZIOK. Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis. European Journal of Pharmaceutical Sciences. Amsterdam: Elsevier, 2024, vol. 193, February, p. 1-15. ISSN 0928-0987. Available from: https://dx.doi.org/10.1016/j.ejps.2023.106683.
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Basic information
Original name Formulation optimization and evaluation of oromucosal in situ gel loaded with silver nanoparticles prepared by green biosynthesis
Authors ROHAĽOVÁ, Simona (703 Slovakia, belonging to the institution), Tomas WOLASCHKA, Ludmila BALAZOVA, Katarina PAULOVICOVA, Jana TOTHOVA, Sylvie PAVLOKOVÁ (203 Czech Republic, belonging to the institution), Martin STAHORSKY and Jan GAJDZIOK (203 Czech Republic, guarantor, belonging to the institution).
Edition European Journal of Pharmaceutical Sciences, Amsterdam, Elsevier, 2024, 0928-0987.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30104 Pharmacology and pharmacy
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.600 in 2022
Organization unit Faculty of Pharmacy
Doi http://dx.doi.org/10.1016/j.ejps.2023.106683
UT WoS 001155785100001
Keywords in English In situ gel; Oral disease; Silver nanoparticles; Agrimonia eupatoria L.; Smart polymer; Principal component analysis
Tags rivok, ÚFT
Tags International impact, Reviewed
Changed by Changed by: JUDr. Sabina Krejčiříková, učo 383857. Changed: 19/2/2024 13:55.
Abstract
Treating oral diseases remains challenging as API is quickly washed out of the application site by saliva turnover and mouth movements. In situ gels are a class of application forms that present sol-gel transition's ability as a response to stimuli. Their tunable properties are provided using smart polymers responsible for stimuli sensitivity, often providing mucoadhesivity. In this study, antimicrobial in situ gels of thermosensitive and pHsensitive polymers loaded with silver nanoparticles were prepared and evaluated. The nanoparticles were prepared by green synthesis using Agrimonia eupatoria L. extract. According to the data analysis, the in situ gel with the most promising profile contained 15 % of Pluronic (R) F-127, 0.25 % of methylcellulose, and 0.1 % of Noveon (R) AA-1. Pluronic (R) F-127 and methylcellulose significantly increased the viscosity of in situ gels at 37 degree celsius and shear rates similar to speaking and swallowing. At 20 degree celsius, a behavior close to a Newtonian fluid was observed while being easily injectable (injection force 13.455 +/- 1.973 N). The viscosity of the formulation increased with temperature and reached 2962.77 +/- 63.37 mPa center dot s (37 degree celsius). A temperature increase led to increased adhesiveness and rigidity of the formulation. The critical sol-gel transition temperature at physiological pH was 32.65 +/- 0.35 degree celsius. 96.77 +/- 3.26 % of Ag NPs were released by erosion and dissolution of the gel after 40 min. The determination of MIC showed effect against E. coli and S. aureus (0.0625 mM and 0.5000 mM, respectively). The relative inhibition zone diameter of the in situ gel was 73.32 +/- 11.06 % compared to gentamicin sulfate. This work discusses the optimization of the formulation of novel antibacterial in situ gel for oromucosal delivery, analyses the impact of the concentration of excipients on the dependent variables, and suggests appropriate evaluation of the formulation in terms of its indication. This study offers a promising dosage form for local treatment of oral diseases.
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