Other formats:
BibTeX
LaTeX
RIS
@article{2364863, author = {Vilím, Jan and Ghazalová, Tereza and Petulová, Eliška and Horackova, Aneta and Stepankova, Veronika and Chaloupková, Radka and Bednář, David and Damborský, Jiří and Prokop, Zbyněk}, article_location = {AMSTERDAM}, article_number = {October 2023}, doi = {http://dx.doi.org/10.1016/j.csbj.2023.10.009}, keywords = {Computer-assisted stabilization; Fibroblast growth factor; Thermostability; Resistance to; Protease; Improved yield; FGF-18}, language = {eng}, issn = {2001-0370}, journal = {Computational and Structural Biotechnology Journal}, title = {Computer-assisted stabilization of fibroblast growth factor FGF-18}, url = {https://www.sciencedirect.com/science/article/pii/S2001037023003665?via%3Dihub}, volume = {21}, year = {2023} }
TY - JOUR ID - 2364863 AU - Vilím, Jan - Ghazalová, Tereza - Petulová, Eliška - Horackova, Aneta - Stepankova, Veronika - Chaloupková, Radka - Bednář, David - Damborský, Jiří - Prokop, Zbyněk PY - 2023 TI - Computer-assisted stabilization of fibroblast growth factor FGF-18 JF - Computational and Structural Biotechnology Journal VL - 21 IS - October 2023 SP - 5144-5152 EP - 5144-5152 PB - Elsevier SN - 20010370 KW - Computer-assisted stabilization KW - Fibroblast growth factor KW - Thermostability KW - Resistance to KW - Protease KW - Improved yield KW - FGF-18 UR - https://www.sciencedirect.com/science/article/pii/S2001037023003665?via%3Dihub N2 - The fibroblast growth factors (FGF) family holds significant potential for addressing chronic diseases. Specifically, recombinant FGF18 shows promise in treating osteoarthritis by stimulating cartilage formation. However, recent phase 2 clinical trial results of sprifermin (recombinant FGF18) indicate insufficient efficacy. Leveraging our expertise in rational protein engineering, we conducted a study to enhance the stability of FGF18. As a result, we obtained a stabilized variant called FGF18-E4, which exhibited improved stability with 16 degrees C higher melting temperature, resistance to trypsin and a 2.5-fold increase in production yields. Moreover, the FGF18-E4 maintained mitogenic activity after 1-week incubation at 37 degrees C and 1-day at 50 degrees C. Additionally, the inserted mutations did not affect its binding to the fibroblast growth factor receptors, making FGF18-E4 a promising candidate for advancing FGF-based osteoarthritis treatment. ER -
VILÍM, Jan, Tereza GHAZALOVÁ, Eliška PETULOVÁ, Aneta HORACKOVA, Veronika STEPANKOVA, Radka CHALOUPKOVÁ, David BEDNÁŘ, Jiří DAMBORSKÝ and Zbyněk PROKOP. Computer-assisted stabilization of fibroblast growth factor FGF-18. \textit{Computational and Structural Biotechnology Journal}. AMSTERDAM: Elsevier, 2023, vol.~21, October 2023, p.~5144-5152. ISSN~2001-0370. Available from: https://dx.doi.org/10.1016/j.csbj.2023.10.009.
|