Next-generation sequencing reveals hidden genomic diversity in
glacial relicts: A case study of Meesia triquetra

J 2024

Next-generation sequencing reveals hidden genomic diversity in glacial relicts: A case study of Meesia triquetra

MIKULÁŠKOVÁ, Eva, Tomáš PETERKA, Jakub ŠMERDA and Michal HÁJEK

Basic information

Original name

Next-generation sequencing reveals hidden genomic diversity in glacial relicts: A case study of Meesia triquetra

Authors

MIKULÁŠKOVÁ, Eva (203 Czech Republic, guarantor, belonging to the institution), Tomáš PETERKA (203 Czech Republic, belonging to the institution), Jakub ŠMERDA (203 Czech Republic, belonging to the institution) and Michal HÁJEK (203 Czech Republic, belonging to the institution)

Edition

Journal of Systematics and Evolution, Hoboken, Wiley, 2024, 1674-4918

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.700 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/jse.13005

UT WoS

001089015500001

Keywords in English

bryophyta; cryptic speciation; endangered moss; Europe; morphometry; north–south gradient; RAD-seq; rich fen

Abstract

V originále

The recent development of genetic methods has facilitated the identification of cryptic species across different groups of organisms, including plants. However, next-generation sequencing has rarely been used to study cryptic speciation in plants, especially in bryophytes, organisms with a dominant haploid life phase. The ability to capture variation across the whole genome makes this method an effective tool for distinguishing cryptic lineages. We have focused on the genetic structure of the moss Meesia triquetra along the Alps-to-Scandinavia transect. We detected the presence of the two genetically critically different lineages of M. triquetra in Europe. These lineages overlap in both morphological characters of the gametophyte and distribution ranges. However, they considerably differ in ecological preferences to groundwater pH. While lineage 1 occupied alkaline to subneutral fens, lineage 2 occurred in fens saturated with neutral to acidic water. We consider the entities cryptic species with respect to genetic and ecological differences but the absence of morphological features necessary for determining the entities. We hypothesize that fragmentation of the ancestral population of the moss in geographically isolated refugia differing in the commonness of acidic and alkaline substrates led to consequent long-term adaptation to different environmental conditions, then drove diversification in M. triquetra.

Links

GJ19-20530Y, research and development project

Name: Současná refugia ohrožených slatiništních rostlin a určující faktory jejich genetické diverzity napříč širokým gradientem propojenosti biotopů (Acronym: Refugia slatiništních rostlin)

Investor: Czech Science Foundation

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MIKULÁŠKOVÁ, Eva, Tomáš PETERKA, Jakub ŠMERDA and Michal HÁJEK. Next-generation sequencing reveals hidden genomic diversity in glacial relicts: A case study of Meesia triquetra. Journal of Systematics and Evolution. Hoboken: Wiley, 2024, vol. 62, No 3, p. 475-488. ISSN 1674-4918. Available from: https://dx.doi.org/10.1111/jse.13005.

@article{2366297,
   author = {Mikulášková, Eva and Peterka, Tomáš and Šmerda, Jakub and Hájek, Michal},
   article_location = {Hoboken},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/jse.13005},
   keywords = {bryophyta; cryptic speciation; endangered moss; Europe; morphometry; north–south gradient; RAD-seq; rich fen},
   language = {eng},
   issn = {1674-4918},
   journal = {Journal of Systematics and Evolution},
   title = {Next-generation sequencing reveals hidden genomic diversity in glacial relicts: A case study of Meesia triquetra},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jse.13005},
   volume = {62},
   year = {2024}
}

TY  - JOUR
ID  - 2366297
AU  - Mikulášková, Eva - Peterka, Tomáš - Šmerda, Jakub - Hájek, Michal
PY  - 2024
TI  - Next-generation sequencing reveals hidden genomic diversity in glacial relicts: A case study of Meesia triquetra
JF  - Journal of Systematics and Evolution
VL  - 62
IS  - 3
SP  - 475-488
EP  - 475-488
PB  - Wiley
SN  - 16744918
KW  - bryophyta
KW  - cryptic speciation
KW  - endangered moss
KW  - Europe
KW  - morphometry
KW  - north–south gradient
KW  - RAD-seq
KW  - rich fen
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/jse.13005
N2  - The recent development of genetic methods has facilitated the identification of cryptic species across different groups of organisms, including plants. However, next-generation sequencing has rarely been used to study cryptic speciation in plants, especially in bryophytes, organisms with a dominant haploid life phase. The ability to capture variation across the whole genome makes this method an effective tool for distinguishing cryptic lineages. We have focused on the genetic structure of the moss Meesia triquetra along the Alps-to-Scandinavia transect. We detected the presence of the two genetically critically different lineages of M. triquetra in Europe. These lineages overlap in both morphological characters of the gametophyte and distribution ranges. However, they considerably differ in ecological preferences to groundwater pH. While lineage 1 occupied alkaline to subneutral fens, lineage 2 occurred in fens saturated with neutral to acidic water. We consider the entities cryptic species with respect to genetic and ecological differences but the absence of morphological features necessary for determining the entities. We hypothesize that fragmentation of the ancestral population of the moss in geographically isolated refugia differing in the commonness of acidic and alkaline substrates led to consequent long-term adaptation to different environmental conditions, then drove diversification in M. triquetra.
ER  -

MIKULÁŠKOVÁ, Eva, Tomáš PETERKA, Jakub ŠMERDA and Michal HÁJEK. Next-generation sequencing reveals hidden genomic diversity in glacial relicts: A case study of Meesia triquetra. \textit{Journal of Systematics and Evolution}. Hoboken: Wiley, 2024, vol.~62, No~3, p.~475-488. ISSN~1674-4918. Available from: https://dx.doi.org/10.1111/jse.13005.

Detailed Information on Publication Record