A color-tunable single-benzene fluorophore-based sensor for
sensitive detection of palladium in solution and living cells

J 2023

A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells

TAKACSOVA, Paulina, Marie KUDLIČKOVÁ PEŠKOVÁ, Pavel SVEC, Zbynek HEGER, Vladimír PEKAŘÍK et. al.

Basic information

Original name

A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells

Authors

TAKACSOVA, Paulina, Marie KUDLIČKOVÁ PEŠKOVÁ (203 Czech Republic, belonging to the institution), Pavel SVEC (203 Czech Republic), Zbynek HEGER (203 Czech Republic) and Vladimír PEKAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analyst, Cambridge, The Royal Society of Chemistry, 2023, 0003-2654

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.200 in 2022

RIV identification code

RIV/00216224:14110/23:00133292

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1039/d3an00046j

UT WoS

000959188700001

Keywords in English

color-tunable single-benzene fluorophore-based; palladium

Abstract

V originále

Single-benzene fluorophores are bright and the smallest fluorochromes known so far. In single-benzene fluorophores, the fluorescence is mediated by the push/pull effect of substituting groups. Despite a plethora of advantageous properties, this group of molecules has not been extensively studied for design of high-performance fluorescent sensors of catalytic or enzymatic activities. Thus, herein, new fluorescent probes based on the Tsuji-Trost reaction were developed for the selective detection of palladium and other transition metals (platinum and gold) in an aqueous/organic mixed solvent with the sensitivity down to 2.5 nM (for palladium). The relative flexibility in the synthesis of these probes allows for facile color tuning of the emitted fluorescence. In this study, we have successfully utilized a yellow emission variant for sensitive detection of palladium under cell-free conditions and in living cells, validating its possible applicability for high-throughput optical sensing of catalysts for bioorthogonal chemistry under physiological conditions.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

TAKACSOVA, Paulina, Marie KUDLIČKOVÁ PEŠKOVÁ, Pavel SVEC, Zbynek HEGER and Vladimír PEKAŘÍK. A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells. Analyst. Cambridge: The Royal Society of Chemistry, 2023, vol. 148, No 9, p. 2058-2063. ISSN 0003-2654. Available from: https://dx.doi.org/10.1039/d3an00046j.

@article{2367148,
   author = {Takacsova, Paulina and Kudličková Pešková, Marie and Svec, Pavel and Heger, Zbynek and Pekařík, Vladimír},
   article_location = {Cambridge},
   article_number = {9},
   doi = {http://dx.doi.org/10.1039/d3an00046j},
   keywords = {color-tunable single-benzene fluorophore-based; palladium},
   language = {eng},
   issn = {0003-2654},
   journal = {Analyst},
   title = {A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells},
   url = {https://pubs.rsc.org/en/content/articlelanding/2023/an/d3an00046j},
   volume = {148},
   year = {2023}
}

TY  - JOUR
ID  - 2367148
AU  - Takacsova, Paulina - Kudličková Pešková, Marie - Svec, Pavel - Heger, Zbynek - Pekařík, Vladimír
PY  - 2023
TI  - A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells
JF  - Analyst
VL  - 148
IS  - 9
SP  - 2058-2063
EP  - 2058-2063
PB  - The Royal Society of Chemistry
SN  - 00032654
KW  - color-tunable single-benzene fluorophore-based
KW  - palladium
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/an/d3an00046j
N2  - Single-benzene fluorophores are bright and the smallest fluorochromes known so far. In single-benzene fluorophores, the fluorescence is mediated by the push/pull effect of substituting groups. Despite a plethora of advantageous properties, this group of molecules has not been extensively studied for design of high-performance fluorescent sensors of catalytic or enzymatic activities. Thus, herein, new fluorescent probes based on the Tsuji-Trost reaction were developed for the selective detection of palladium and other transition metals (platinum and gold) in an aqueous/organic mixed solvent with the sensitivity down to 2.5 nM (for palladium). The relative flexibility in the synthesis of these probes allows for facile color tuning of the emitted fluorescence. In this study, we have successfully utilized a yellow emission variant for sensitive detection of palladium under cell-free conditions and in living cells, validating its possible applicability for high-throughput optical sensing of catalysts for bioorthogonal chemistry under physiological conditions.
ER  -

TAKACSOVA, Paulina, Marie KUDLIČKOVÁ PEŠKOVÁ, Pavel SVEC, Zbynek HEGER and Vladimír PEKAŘÍK. A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells. \textit{Analyst}. Cambridge: The Royal Society of Chemistry, 2023, vol.~148, No~9, p.~2058-2063. ISSN~0003-2654. Available from: https://dx.doi.org/10.1039/d3an00046j.

Detailed Information on Publication Record