An Overview of Systematic Reviews of Polymerase Chain Reaction
(PCR) for the Diagnosis of Invasive Aspergillosis in
Immunocompromised People: A Report of the Fungal PCR Initiative
(FPCRI)-An ISHAM Working Group

J 2023

An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)-An ISHAM Working Group

CRUCIANI, Mario, P Lewis WHITE, Rosemary A BARNES, Juergen LOEFFLER, J Peter DONNELLY et. al.

Basic information

Original name

An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)-An ISHAM Working Group

Authors

CRUCIANI, Mario (guarantor), P Lewis WHITE, Rosemary A BARNES, Juergen LOEFFLER, J Peter DONNELLY, Thomas R ROGERS, Werner J HEINZ, Adilia WARRIS, Charles Oliver MORTON, Martina LENGEROVÁ (203 Czech Republic, belonging to the institution), Lena KLINGSPOR, Boualem SENDID and Deborah E A LOCKHART

Edition

Journal of Fungi, St. Alban-Anlage, MDPI, 2023, 2309-608X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10612 Mycology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.700 in 2022

RIV identification code

RIV/00216224:14740/23:00133294

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/jof9100967

UT WoS

001099510500001

Keywords in English

systematic review; meta-analysis; umbrella review; Aspergillus fumigatus; invasive aspergillosis; diagnosis; polymerase chain reaction (PCR)

Abstract

V originále

This overview of reviews (i.e., an umbrella review) is designed to reappraise the validity of systematic reviews (SRs) and meta-analyses related to the performance of Aspergillus PCR tests for the diagnosis of invasive aspergillosis in immunocompromised patients. The methodological quality of the SRs was assessed using the AMSTAR-2 checklist; the quality of the evidence (QOE) within each SR was appraised following the GRADE approach. Eight out of 12 SRs were evaluated for qualitative and quantitative assessment. Five SRs evaluated Aspergillus PCR on bronchoalveolar lavage fluid (BAL) and three on blood specimens. The eight SRs included 167 overlapping reports (59 evaluating PCR in blood specimens, and 108 in BAL), based on 107 individual primary studies (98 trials with a cohort design, and 19 with a case-control design). In BAL specimens, the mean sensitivity and specificity ranged from 0.57 to 0.91, and from 0.92 to 0.97, respectively (QOE: very low to low). In blood specimens (whole blood or serum), the mean sensitivity ranged from 0.57 to 0.84, and the mean specificity from 0.58 to 0.95 (QOE: low to moderate). Across studies, only a low proportion of AMSTAR-2 critical domains were unmet (1.8%), demonstrating a high quality of methodological assessment. Conclusions. Based on the overall methodological assessment of the reviews included, on average we can have high confidence in the quality of results generated by the SRs.

Citovat

CRUCIANI, Mario, P Lewis WHITE, Rosemary A BARNES, Juergen LOEFFLER, J Peter DONNELLY, Thomas R ROGERS, Werner J HEINZ, Adilia WARRIS, Charles Oliver MORTON, Martina LENGEROVÁ, Lena KLINGSPOR, Boualem SENDID and Deborah E A LOCKHART. An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)-An ISHAM Working Group. Journal of Fungi. St. Alban-Anlage: MDPI, 2023, vol. 9, No 10, p. 1-15. ISSN 2309-608X. Available from: https://dx.doi.org/10.3390/jof9100967.

@article{2367156,
   author = {Cruciani, Mario and White, P Lewis and Barnes, Rosemary A and Loeffler, Juergen and Donnelly, J Peter and Rogers, Thomas R and Heinz, Werner J and Warris, Adilia and Morton, Charles Oliver and Lengerová, Martina and Klingspor, Lena and Sendid, Boualem and Lockhart, Deborah E A},
   article_location = {St. Alban-Anlage},
   article_number = {10},
   doi = {http://dx.doi.org/10.3390/jof9100967},
   keywords = {systematic review; meta-analysis; umbrella review; Aspergillus fumigatus; invasive aspergillosis; diagnosis; polymerase chain reaction (PCR)},
   language = {eng},
   issn = {2309-608X},
   journal = {Journal of Fungi},
   title = {An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)-An ISHAM Working Group},
   url = {https://www.mdpi.com/2309-608X/9/10/967},
   volume = {9},
   year = {2023}
}

TY  - JOUR
ID  - 2367156
AU  - Cruciani, Mario - White, P Lewis - Barnes, Rosemary A - Loeffler, Juergen - Donnelly, J Peter - Rogers, Thomas R - Heinz, Werner J - Warris, Adilia - Morton, Charles Oliver - Lengerová, Martina - Klingspor, Lena - Sendid, Boualem - Lockhart, Deborah E A
PY  - 2023
TI  - An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)-An ISHAM Working Group
JF  - Journal of Fungi
VL  - 9
IS  - 10
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 2309608X
KW  - systematic review
KW  - meta-analysis
KW  - umbrella review
KW  - Aspergillus fumigatus
KW  - invasive aspergillosis
KW  - diagnosis
KW  - polymerase chain reaction (PCR)
UR  - https://www.mdpi.com/2309-608X/9/10/967
N2  - This overview of reviews (i.e., an umbrella review) is designed to reappraise the validity of systematic reviews (SRs) and meta-analyses related to the performance of Aspergillus PCR tests for the diagnosis of invasive aspergillosis in immunocompromised patients. The methodological quality of the SRs was assessed using the AMSTAR-2 checklist; the quality of the evidence (QOE) within each SR was appraised following the GRADE approach. Eight out of 12 SRs were evaluated for qualitative and quantitative assessment. Five SRs evaluated Aspergillus PCR on bronchoalveolar lavage fluid (BAL) and three on blood specimens. The eight SRs included 167 overlapping reports (59 evaluating PCR in blood specimens, and 108 in BAL), based on 107 individual primary studies (98 trials with a cohort design, and 19 with a case-control design). In BAL specimens, the mean sensitivity and specificity ranged from 0.57 to 0.91, and from 0.92 to 0.97, respectively (QOE: very low to low). In blood specimens (whole blood or serum), the mean sensitivity ranged from 0.57 to 0.84, and the mean specificity from 0.58 to 0.95 (QOE: low to moderate). Across studies, only a low proportion of AMSTAR-2 critical domains were unmet (1.8%), demonstrating a high quality of methodological assessment. Conclusions. Based on the overall methodological assessment of the reviews included, on average we can have high confidence in the quality of results generated by the SRs.
ER  -

CRUCIANI, Mario, P Lewis WHITE, Rosemary A BARNES, Juergen LOEFFLER, J Peter DONNELLY, Thomas R ROGERS, Werner J HEINZ, Adilia WARRIS, Charles Oliver MORTON, Martina LENGEROVÁ, Lena KLINGSPOR, Boualem SENDID and Deborah E A LOCKHART. An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)-An ISHAM Working Group. \textit{Journal of Fungi}. St. Alban-Anlage: MDPI, 2023, vol.~9, No~10, p.~1-15. ISSN~2309-608X. Available from: https://dx.doi.org/10.3390/jof9100967.

Detailed Information on Publication Record