The Lucigenin Assay: Measuring Anion Transport in Lipid
Vesicles

J 2024

The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles

CHVOJKA, Matúš, Anurag SINGH, Alessio CATALDO, Aaron TORRES-HUERTA, Marcin KONOPKA et. al.

Basic information

Original name

The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles

Authors

CHVOJKA, Matúš (703 Slovakia, belonging to the institution), Anurag SINGH, Alessio CATALDO, Aaron TORRES-HUERTA, Marcin KONOPKA, Vladimír ŠINDELÁŘ (203 Czech Republic, belonging to the institution) and Hennie VALKENIER (guarantor)

Edition

Analysis and Sensing, Wiley, 2024, 2629-2742

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10401 Organic chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/anse.202300044

UT WoS

001119196500001

Keywords in English

anion transport; fluorescent probes; fluorescence spectroscopy; liposomes; supramolecular chemistry

Abstract

V originále

Synthetic anion transporters are developed to transport anions across lipid membranes with the long-term perspective of biological applications. The lucigenin assay is a popular tool to study their transport of chloride and other anions in liposomes. It relies on the quenching of the fluorescence of encapsulated lucigenin by anions, which can be monitored by fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay. It describes in detail how to use this assay to monitor chloride/nitrate antiport in liposomes, process and interpret the data, and solve common issues. Variations of the assay enabling the investigation of the transport of other anions and transport mechanisms are discussed. Furthermore, a zwitterionic analogue of lucigenin is demonstrated to have advantages for use in experiments over longer time scales, as it does not leak out of the liposomes, or when studying chloride uniport, as it avoids interference from antiport with nitrate that is present in commercial lucigenin.

Links

MUNI/A/1209/2022, interní kód MU

Name: Molekuly, komplexy, makrocykly a xerogely

Investor: Masaryk University, Molecules, complexes, macrocycles, and xerogels

Citovat

CHVOJKA, Matúš, Anurag SINGH, Alessio CATALDO, Aaron TORRES-HUERTA, Marcin KONOPKA, Vladimír ŠINDELÁŘ and Hennie VALKENIER. The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles. Analysis and Sensing. Wiley, 2024, vol. 4, No 2, p. e20230004, 16 pp. ISSN 2629-2742. Available from: https://dx.doi.org/10.1002/anse.202300044.

@article{2367858,
   author = {Chvojka, Matúš and Singh, Anurag and Cataldo, Alessio and TorresandHuerta, Aaron and Konopka, Marcin and Šindelář, Vladimír and Valkenier, Hennie},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/anse.202300044},
   keywords = {anion transport; fluorescent probes; fluorescence spectroscopy; liposomes; supramolecular chemistry},
   language = {eng},
   issn = {2629-2742},
   journal = {Analysis and Sensing},
   title = {The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles},
   url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/anse.202300044},
   volume = {4},
   year = {2024}
}

TY  - JOUR
ID  - 2367858
AU  - Chvojka, Matúš - Singh, Anurag - Cataldo, Alessio - Torres-Huerta, Aaron - Konopka, Marcin - Šindelář, Vladimír - Valkenier, Hennie
PY  - 2024
TI  - The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles
JF  - Analysis and Sensing
VL  - 4
IS  - 2
SP  - e20230004
EP  - e20230004
PB  - Wiley
SN  - 26292742
KW  - anion transport
KW  - fluorescent probes
KW  - fluorescence spectroscopy
KW  - liposomes
KW  - supramolecular chemistry
UR  - https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/anse.202300044
N2  - Synthetic anion transporters are developed to transport anions across lipid membranes with the long-term perspective of biological applications. The lucigenin assay is a popular tool to study their transport of chloride and other anions in liposomes. It relies on the quenching of the fluorescence of encapsulated lucigenin by anions, which can be monitored by fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay. It describes in detail how to use this assay to monitor chloride/nitrate antiport in liposomes, process and interpret the data, and solve common issues. Variations of the assay enabling the investigation of the transport of other anions and transport mechanisms are discussed. Furthermore, a zwitterionic analogue of lucigenin is demonstrated to have advantages for use in experiments over longer time scales, as it does not leak out of the liposomes, or when studying chloride uniport, as it avoids interference from antiport with nitrate that is present in commercial lucigenin.
ER  -

CHVOJKA, Matúš, Anurag SINGH, Alessio CATALDO, Aaron TORRES-HUERTA, Marcin KONOPKA, Vladimír ŠINDELÁŘ and Hennie VALKENIER. The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles. \textit{Analysis and Sensing}. Wiley, 2024, vol.~4, No~2, p.~e20230004, 16 pp. ISSN~2629-2742. Available from: https://dx.doi.org/10.1002/anse.202300044.

Detailed Information on Publication Record