| CHVOJKA, Matúš, Anurag SINGH, Alessio CATALDO, Aaron TORRES-HUERTA, Marcin KONOPKA, Vladimír ŠINDELÁŘ and Hennie VALKENIER. The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles. Analysis and Sensing. Wiley, 2024, vol. 4, No 2, p. e20230004, 16 pp. ISSN 2629-2742. Available from: https://dx.doi.org/10.1002/anse.202300044. |
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@article{2367858,
author = {Chvojka, Matúš and Singh, Anurag and Cataldo, Alessio and TorresandHuerta, Aaron and Konopka, Marcin and Šindelář, Vladimír and Valkenier, Hennie},
article_number = {2},
doi = {http://dx.doi.org/10.1002/anse.202300044},
keywords = {anion transport; fluorescent probes; fluorescence spectroscopy; liposomes; supramolecular chemistry},
language = {eng},
issn = {2629-2742},
journal = {Analysis and Sensing},
title = {The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles},
url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/anse.202300044},
volume = {4},
year = {2024}
}
TY - JOUR
ID - 2367858
AU - Chvojka, Matúš - Singh, Anurag - Cataldo, Alessio - Torres-Huerta, Aaron - Konopka, Marcin - Šindelář, Vladimír - Valkenier, Hennie
PY - 2024
TI - The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles
JF - Analysis and Sensing
VL - 4
IS - 2
SP - e20230004
EP - e20230004
PB - Wiley
SN - 26292742
KW - anion transport
KW - fluorescent probes
KW - fluorescence spectroscopy
KW - liposomes
KW - supramolecular chemistry
UR - https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/anse.202300044
N2 - Synthetic anion transporters are developed to transport anions across lipid membranes with the long-term perspective of biological applications. The lucigenin assay is a popular tool to study their transport of chloride and other anions in liposomes. It relies on the quenching of the fluorescence of encapsulated lucigenin by anions, which can be monitored by fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay. It describes in detail how to use this assay to monitor chloride/nitrate antiport in liposomes, process and interpret the data, and solve common issues. Variations of the assay enabling the investigation of the transport of other anions and transport mechanisms are discussed. Furthermore, a zwitterionic analogue of lucigenin is demonstrated to have advantages for use in experiments over longer time scales, as it does not leak out of the liposomes, or when studying chloride uniport, as it avoids interference from antiport with nitrate that is present in commercial lucigenin.
ER -
CHVOJKA, Matúš, Anurag SINGH, Alessio CATALDO, Aaron TORRES-HUERTA, Marcin KONOPKA, Vladimír ŠINDELÁŘ and Hennie VALKENIER. The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles. \textit{Analysis and Sensing}. Wiley, 2024, vol.~4, No~2, p.~e20230004, 16 pp. ISSN~2629-2742. Available from: https://dx.doi.org/10.1002/anse.202300044.
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