| MIČAN, Jan, Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO, Uwe T. BORNSCHEUER, Jiří DAMBORSKÝ, Ren WEI and David BEDNÁŘ. Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes. Applied Catalysis B: Environment and Energy. Amsterdam: Elsevier, 2024, vol. 342, March 2024, p. 1-16. ISSN 0926-3373. Available from: https://dx.doi.org/10.1016/j.apcatb.2023.123404. |
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@article{2367868,
author = {Mičan, Jan and Jaradat, Da 'san M. M. and Liu, Weidong and Weber, Gert and Mazurenko, Stanislav and Bornscheuer, Uwe T. and Damborský, Jiří and Wei, Ren and Bednář, David},
article_location = {Amsterdam},
article_number = {March 2024},
doi = {http://dx.doi.org/10.1016/j.apcatb.2023.123404},
keywords = {PET recycling; Protein engineering; Enzymatic degradation; Microbial dark matter; AlphaFold; High -throughput screening},
language = {eng},
issn = {0926-3373},
journal = {Applied Catalysis B: Environment and Energy},
note = {LF žádný přínos dle informací od doc. Bednáře.},
title = {Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes},
url = {https://www.sciencedirect.com/science/article/pii/S0926337323010470?via%3Dihub},
volume = {342},
year = {2024}
}
TY - JOUR
ID - 2367868
AU - Mičan, Jan - Jaradat, Da 'san M. M. - Liu, Weidong - Weber, Gert - Mazurenko, Stanislav - Bornscheuer, Uwe T. - Damborský, Jiří - Wei, Ren - Bednář, David
PY - 2024
TI - Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes
JF - Applied Catalysis B: Environment and Energy
VL - 342
IS - March 2024
SP - 1-16
EP - 1-16
PB - Elsevier
SN - 09263373
N1 - LF žádný přínos dle informací od doc. Bednáře.
KW - PET recycling
KW - Protein engineering
KW - Enzymatic degradation
KW - Microbial dark matter
KW - AlphaFold
KW - High -throughput screening
UR - https://www.sciencedirect.com/science/article/pii/S0926337323010470?via%3Dihub
N2 - Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material properties and low cost. However, PET contributes significantly to the growing problem of plastic waste pollution. Enzymatic PET recycling has emerged as a promising alternative to conventional mechanical and chemical recycling methods. While many PET hydrolases - belonging to the alpha/beta-hydrolase fold superfamily - have been discovered, the wild -type enzymes obtained from natural sources are not optimal for industrial conditions and need to be optimized through rational design or directed evolution to improve their efficiency and stability. This Perspective sum-marizes case studies of engineered PET hydrolases and proposes a workflow that tightly integrates a variety of in silico and high-throughput approaches for biochemical and structural characterization to accelerate the discovery of PET-degrading enzymes, also with novel structural scaffolds. These biocatalysts could be candidates for developing further innovative plastic recycling techniques.
ER -
MIČAN, Jan, Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO, Uwe T. BORNSCHEUER, Jiří DAMBORSKÝ, Ren WEI and David BEDNÁŘ. Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes. \textit{Applied Catalysis B: Environment and Energy}. Amsterdam: Elsevier, 2024, vol.~342, March 2024, p.~1-16. ISSN~0926-3373. Available from: https://dx.doi.org/10.1016/j.apcatb.2023.123404.
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