J 2024

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

MIČAN, Jan, Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO et. al.

Basic information

Original name

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

Authors

MIČAN, Jan (203 Czech Republic, belonging to the institution), Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO (643 Russian Federation, belonging to the institution), Uwe T. BORNSCHEUER, Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution), Ren WEI and David BEDNÁŘ (203 Czech Republic, belonging to the institution)

Edition

Applied Catalysis B: Environment and Energy, Amsterdam, Elsevier, 2024, 0926-3373

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 22.100 in 2022

Organization unit

Faculty of Science

UT WoS

001110359200001

Keywords in English

PET recycling; Protein engineering; Enzymatic degradation; Microbial dark matter; AlphaFold; High -throughput screening

Tags

Tags

International impact, Reviewed
Změněno: 5/3/2024 10:36, Mgr. Marie Šípková, DiS.

Abstract

V originále

Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material properties and low cost. However, PET contributes significantly to the growing problem of plastic waste pollution. Enzymatic PET recycling has emerged as a promising alternative to conventional mechanical and chemical recycling methods. While many PET hydrolases - belonging to the alpha/beta-hydrolase fold superfamily - have been discovered, the wild -type enzymes obtained from natural sources are not optimal for industrial conditions and need to be optimized through rational design or directed evolution to improve their efficiency and stability. This Perspective sum-marizes case studies of engineered PET hydrolases and proposes a workflow that tightly integrates a variety of in silico and high-throughput approaches for biochemical and structural characterization to accelerate the discovery of PET-degrading enzymes, also with novel structural scaffolds. These biocatalysts could be candidates for developing further innovative plastic recycling techniques.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
FW03010208, research and development project
Name: Stabilizace aplikačně atraktivních FGF proteinů pomocí pokročilých automatizovaných metod proteinového inženýrství
Investor: Technology Agency of the Czech Republic, Subprograms 1 Technology leaders
LM2023055, research and development project
Name: Česká národní infrastruktura pro biologická data
Investor: Ministry of Education, Youth and Sports of the CR, ELIXIR-CZ: Czech National Infrastructure for Biological Data
LM2023069, research and development project
Name: Výzkumná infrastruktura RECETOX
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure
857560, interní kód MU
(CEP code: EF17_043/0009632)
Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)
Investor: European Union, Spreading excellence and widening participation