Arabidopsis flippase ALA3 is required for adjustment of early
subcellular trafficking in plant response to osmotic stress

J 2023

Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress

PUKYŠOVÁ, Vendula, Adria SANS SÁNCHEZ, Jiří RUDOLF, Tomasz NODZYNSKI, Marta ZWIEWKA et. al.

Basic information

Original name

Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress

Authors

PUKYŠOVÁ, Vendula (203 Czech Republic, belonging to the institution), Adria SANS SÁNCHEZ (724 Spain, belonging to the institution), Jiří RUDOLF (203 Czech Republic, belonging to the institution), Tomasz NODZYNSKI (616 Poland, belonging to the institution) and Marta ZWIEWKA (616 Poland, guarantor, belonging to the institution)

Edition

Journal of Experimental Botany, Oxford, Oxford University Press, 2023, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.900 in 2022

RIV identification code

RIV/00216224:14740/23:00133365

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/jxb/erad234

UT WoS

001022967500001

Keywords in English

Arabidopsis thaliana; ARF; endocytosis; flippase; GEF; osmotic stress; protein trafficking; secretion

Abstract

V originále

To compensate for their sessile lifestyle, plants developed several responses to exogenous changes. One of the previously investigated and not yet fully understood adaptations occurs at the level of early subcellular trafficking, which needs to be rapidly adjusted to maintain cellular homeostasis and membrane integrity under osmotic stress conditions. To form a vesicle, the membrane needs to be deformed, which is ensured by multiple factors, including the activity of specific membrane proteins, such as flippases from the family of P4-ATPases. The membrane pumps actively translocate phospholipids from the exoplasmic/luminal to the cytoplasmic membrane leaflet to generate curvature, which might be coupled with recruitment of proteins involved in vesicle formation at specific sites of the donor membrane. We show that lack of the AMINOPHOSPHOLIPID ATPASE3 (ALA3) flippase activity caused defects at the plasma membrane and trans-Golgi network, resulting in altered endocytosis and secretion, processes relying on vesicle formation and movement. The mentioned cellular defects were translated into decreased intracellular trafficking flexibility failing to adjust the root growth on osmotic stress-eliciting media. In conclusion, we show that ALA3 cooperates with ARF-GEF BIG5/BEN1 and ARF1A1C/BEX1 in a similar regulatory pathway to vesicle formation, and together they are important for plant adaptation to osmotic stress. The interplay of P4-ATPase ALA3, ARF BEX1, and ARF-GEF BEN1 controls vesicle formation at the plasma membrane/trans -Golgi network and the subsequent vesicle trafficking important for the plant response to osmotic stress.

Links

GJ20-20860Y, research and development project

Name: Odhalení nových regulátorů vnitrobuněčného transportu zapojených do stresové reakce rostlin.

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90250, large research infrastructures

Name: Czech-BioImaging III

Citovat

PUKYŠOVÁ, Vendula, Adria SANS SÁNCHEZ, Jiří RUDOLF, Tomasz NODZYNSKI and Marta ZWIEWKA. Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress. Journal of Experimental Botany. Oxford: Oxford University Press, 2023, vol. 74, No 17, p. 4959-4977. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erad234.

@article{2368521,
   author = {Pukyšová, Vendula and Sans Sánchez, Adria and Rudolf, Jiří and Nodzynski, Tomasz and Zwiewka, Marta},
   article_location = {Oxford},
   article_number = {17},
   doi = {http://dx.doi.org/10.1093/jxb/erad234},
   keywords = {Arabidopsis thaliana; ARF; endocytosis; flippase; GEF; osmotic stress; protein trafficking; secretion},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress},
   url = {https://academic.oup.com/jxb/article/74/17/4959/7206353},
   volume = {74},
   year = {2023}
}

TY  - JOUR
ID  - 2368521
AU  - Pukyšová, Vendula - Sans Sánchez, Adria - Rudolf, Jiří - Nodzynski, Tomasz - Zwiewka, Marta
PY  - 2023
TI  - Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress
JF  - Journal of Experimental Botany
VL  - 74
IS  - 17
SP  - 4959-4977
EP  - 4959-4977
PB  - Oxford University Press
SN  - 00220957
KW  - Arabidopsis thaliana
KW  - ARF
KW  - endocytosis
KW  - flippase
KW  - GEF
KW  - osmotic stress
KW  - protein trafficking
KW  - secretion
UR  - https://academic.oup.com/jxb/article/74/17/4959/7206353
N2  - To compensate for their sessile lifestyle, plants developed several responses to exogenous changes. One of the previously investigated and not yet fully understood adaptations occurs at the level of early subcellular trafficking, which needs to be rapidly adjusted to maintain cellular homeostasis and membrane integrity under osmotic stress conditions. To form a vesicle, the membrane needs to be deformed, which is ensured by multiple factors, including the activity of specific membrane proteins, such as flippases from the family of P4-ATPases. The membrane pumps actively translocate phospholipids from the exoplasmic/luminal to the cytoplasmic membrane leaflet to generate curvature, which might be coupled with recruitment of proteins involved in vesicle formation at specific sites of the donor membrane. We show that lack of the AMINOPHOSPHOLIPID ATPASE3 (ALA3) flippase activity caused defects at the plasma membrane and trans-Golgi network, resulting in altered endocytosis and secretion, processes relying on vesicle formation and movement. The mentioned cellular defects were translated into decreased intracellular trafficking flexibility failing to adjust the root growth on osmotic stress-eliciting media. In conclusion, we show that ALA3 cooperates with ARF-GEF BIG5/BEN1 and ARF1A1C/BEX1 in a similar regulatory pathway to vesicle formation, and together they are important for plant adaptation to osmotic stress. The interplay of P4-ATPase ALA3, ARF BEX1, and ARF-GEF BEN1 controls vesicle formation at the plasma membrane/trans -Golgi network and the subsequent vesicle trafficking important for the plant response to osmotic stress.
ER  -

PUKYŠOVÁ, Vendula, Adria SANS SÁNCHEZ, Jiří RUDOLF, Tomasz NODZYNSKI and Marta ZWIEWKA. Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress. \textit{Journal of Experimental Botany}. Oxford: Oxford University Press, 2023, vol.~74, No~17, p.~4959-4977. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erad234.

Detailed Information on Publication Record