Listeria monocytogenes clones circulating in the natural
environment of the Czech Republic and Slovakia

J 2023

Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia

TOMASTIKOVA, Zuzana, Lucie HLUCHANOVA, Tereza GELBÍČOVÁ and Renata KARPÍŠKOVÁ

Basic information

Original name

Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia

Authors

TOMASTIKOVA, Zuzana (203 Czech Republic), Lucie HLUCHANOVA (203 Czech Republic, guarantor), Tereza GELBÍČOVÁ (203 Czech Republic, belonging to the institution) and Renata KARPÍŠKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Czech Journal of Food Sciences, Praha, ČR, Czech Academy of Agricultural Sciences, 2023, 1212-1800

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.300 in 2022

RIV identification code

RIV/00216224:14110/23:00133381

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.17221/234/2022-CJFS

UT WoS

000974395300001

Keywords in English

nature; serotyping; macrorestriction analysis; antimicrobial susceptibility; whole genome sequencing

Abstract

V originále

Listeria monocytogenes is not only a pathogen causing a serious food-borne disease in humans but can also occur as a saprophyte in the natural environment. This study aimed to evaluate the occurrence of L. monocytogenes obtained from the natural environment of the Czech Republic (hereinafter Czechia) and Slovakia in 2016-2018 and to compare the clonal relationship of strains circulating in the environment with the strains originating from the food chain and humans. Altogether, 217 samples of mud, surface water, vegetation and soil were collected in 61 locations. Samples were processed according to the modified EN ISO 11290-1 standard. The obtained L. monocytogenes isolates were characterised using serotyping, macrorestriction analysis, followed by pulsed-field gel electrophoresis, whole genome sequencing (WGS), and antimicrobial susceptibility testing. L. monocytogenes were detected in 8.8% of the examined samples and were isolated in 15 locations, mainly from the mud from the banks of the surface water sources. Altogether, 25 L. monocytogenes strains were obtained from 19 positive samples. Serotypes 1/2a, 4b, and 1/2b were detected among the strains. Twenty combined AscI/ApaI pulsotypes were obtained by macrorestriction analysis. Altogether, 12 sequence types (STs) were detected using Multi-locus sequence typing (MLST) by WGS, with ST451 being the most frequent. The core genome MLST analysis revealed a heterogeneous population of environmental strains. No phenotype resistance was detected by antimicrobial susceptibility testing. Screening of antimicrobial-resistance genes using the platform ResFinder revealed the genes fosX in 24 isolates and blaTEM-116 in one isolate. The occurrence of L. monocytogenes in various samples from natural environments within wide altitude range during different seasons of the year may highlight this bacterium's remarkable adaptability and exceptional tolerance to external factors. Serotype distribution of the strains circulating in the natural environment of Czechia and Slovakia seems to reflect distribution in the human population more than in the food chain.

Citovat

TOMASTIKOVA, Zuzana, Lucie HLUCHANOVA, Tereza GELBÍČOVÁ and Renata KARPÍŠKOVÁ. Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia. Czech Journal of Food Sciences. Praha, ČR: Czech Academy of Agricultural Sciences, 2023, vol. 41, No 2, p. 127-136. ISSN 1212-1800. Available from: https://dx.doi.org/10.17221/234/2022-CJFS.

@article{2368628,
   author = {Tomastikova, Zuzana and Hluchanova, Lucie and Gelbíčová, Tereza and Karpíšková, Renata},
   article_location = {Praha, ČR},
   article_number = {2},
   doi = {http://dx.doi.org/10.17221/234/2022-CJFS},
   keywords = {nature; serotyping; macrorestriction analysis; antimicrobial susceptibility; whole genome sequencing},
   language = {eng},
   issn = {1212-1800},
   journal = {Czech Journal of Food Sciences},
   title = {Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia},
   url = {https://cjfs.agriculturejournals.cz/artkey/cjf-202302-0006_listeria-monocytogenes-clones-circulating-in-the-natural-environment-of-the-czech-republic-and-slovakia.php},
   volume = {41},
   year = {2023}
}

TY  - JOUR
ID  - 2368628
AU  - Tomastikova, Zuzana - Hluchanova, Lucie - Gelbíčová, Tereza - Karpíšková, Renata
PY  - 2023
TI  - Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia
JF  - Czech Journal of Food Sciences
VL  - 41
IS  - 2
SP  - 127-136
EP  - 127-136
PB  - Czech Academy of Agricultural Sciences
SN  - 12121800
KW  - nature
KW  - serotyping
KW  - macrorestriction analysis
KW  - antimicrobial susceptibility
KW  - whole genome sequencing
UR  - https://cjfs.agriculturejournals.cz/artkey/cjf-202302-0006_listeria-monocytogenes-clones-circulating-in-the-natural-environment-of-the-czech-republic-and-slovakia.php
N2  - Listeria monocytogenes is not only a pathogen causing a serious food-borne disease in humans but can also occur as a saprophyte in the natural environment. This study aimed to evaluate the occurrence of L. monocytogenes obtained from the natural environment of the Czech Republic (hereinafter Czechia) and Slovakia in 2016-2018 and to compare the clonal relationship of strains circulating in the environment with the strains originating from the food chain and humans. Altogether, 217 samples of mud, surface water, vegetation and soil were collected in 61 locations. Samples were processed according to the modified EN ISO 11290-1 standard. The obtained L. monocytogenes isolates were characterised using serotyping, macrorestriction analysis, followed by pulsed-field gel electrophoresis, whole genome sequencing (WGS), and antimicrobial susceptibility testing. L. monocytogenes were detected in 8.8% of the examined samples and were isolated in 15 locations, mainly from the mud from the banks of the surface water sources. Altogether, 25 L. monocytogenes strains were obtained from 19 positive samples. Serotypes 1/2a, 4b, and 1/2b were detected among the strains. Twenty combined AscI/ApaI pulsotypes were obtained by macrorestriction analysis. Altogether, 12 sequence types (STs) were detected using Multi-locus sequence typing (MLST) by WGS, with ST451 being the most frequent. The core genome MLST analysis revealed a heterogeneous population of environmental strains. No phenotype resistance was detected by antimicrobial susceptibility testing. Screening of antimicrobial-resistance genes using the platform ResFinder revealed the genes fosX in 24 isolates and blaTEM-116 in one isolate. The occurrence of L. monocytogenes in various samples from natural environments within wide altitude range during different seasons of the year may highlight this bacterium's remarkable adaptability and exceptional tolerance to external factors. Serotype distribution of the strains circulating in the natural environment of Czechia and Slovakia seems to reflect distribution in the human population more than in the food chain.
ER  -

TOMASTIKOVA, Zuzana, Lucie HLUCHANOVA, Tereza GELBÍČOVÁ and Renata KARPÍŠKOVÁ. Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia. \textit{Czech Journal of Food Sciences}. Praha, ČR: Czech Academy of Agricultural Sciences, 2023, vol.~41, No~2, p.~127-136. ISSN~1212-1800. Available from: https://dx.doi.org/10.17221/234/2022-CJFS.

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