Escherichia coli from Human Wounds: Analysis of Resistance to
β-Lactams and Expression of RND Efflux Pumps

J 2023

Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps

RIHACEK, Martin, Michaela KUTHANOVA, Zbynek SPLICHAL, Vojtech ADAM, Kristyna HRAZDILOVA et. al.

Basic information

Original name

Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps

Authors

RIHACEK, Martin (203 Czech Republic), Michaela KUTHANOVA (203 Czech Republic), Zbynek SPLICHAL (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Kristyna HRAZDILOVA (203 Czech Republic), Radek VESELÝ (203 Czech Republic, belonging to the institution), Ludek ZUREK (203 Czech Republic) and Kristyna CIHALOVA (203 Czech Republic, guarantor)

Edition

INFECTION AND DRUG RESISTANCE, AUCKLAND, DOVE MEDICAL PRESS LTD, 2023, 1178-6973

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30230 Other clinical medicine subjects

Country of publisher

New Zealand

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.900 in 2022

RIV identification code

RIV/00216224:14110/23:00133383

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.2147/IDR.S435622

UT WoS

001113170700001

Keywords in English

clinical isolates; ampicillin resistance; resistance mechanisms; antibiotics

Abstract

V originále

Purpose: Resistance of pathogenic strains of Escherichia coli to beta-lactams, particularly to ampicillin, is on the rise and it is attributed to intrinsic and acquired mechanisms. One important factor contributing to resistance, together with primarily resistance mechanisms, is a mutation and/or an over-expression of the intrinsic efflux pumps in the resistance-nodulation-division (RND) superfamily. Among these efflux pumps, AcrA, AcrB, TolC, and AcrD play an important role in antimicrobial co-resistance, including resistance to beta-lactams.Materials and Methods: Twelve E. coli isolates obtained from patients' wounds and the control strain of E. coli ATCC 25922 were analyzed. The phenotypic resistance of these isolates to selected beta-lactams was assessed by determination of the minimal inhibitory concentration. Additionally, the prevalence of beta-lactamase genes (blaTEM, blaCTX-M, blaSHV, and blaAmpC) was screened by PCR. Realtime qPCR was used to determine the expression of the selected efflux pumps acrA, acrB, tolC, and acrD and the repressor acrR after the exposure of E. coli to ampicillin.Results: Phenotypic resistance to beta-lactams was detected in seven isolates, mainly to ampicillin and piperacillin. This was corroborated by the presence of at least one acquired bla gene in each of these isolates. Although E. coli strains varied in the expression of RND-family efflux pumps after the ampicillin exposure, their gene expression indicated that these pumps did not play a major role in the phenotypic resistance to ampicillin.Conclusion: Each E. coli isolate displayed unique characteristics, differing in minimum inhibitory concentration (MIC) values, prevalence of acquired blaTEM and blaCTX-M genes, and expression of the RND-family pumps. This together demonstrates that these clinical isolates employed distinct intrinsic or acquired resistance pathways for their defense against ampicillin. The prevalence and spread of ampicillin resistant E. coli has to be monitored and the search for ampicillin alternatives is needed.

Citovat

RIHACEK, Martin, Michaela KUTHANOVA, Zbynek SPLICHAL, Vojtech ADAM, Kristyna HRAZDILOVA, Radek VESELÝ, Ludek ZUREK and Kristyna CIHALOVA. Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps. INFECTION AND DRUG RESISTANCE. AUCKLAND: DOVE MEDICAL PRESS LTD, 2023, vol. 16, November 2023, p. 7365-7375. ISSN 1178-6973. Available from: https://dx.doi.org/10.2147/IDR.S435622.

@article{2368638,
   author = {Rihacek, Martin and Kuthanova, Michaela and Splichal, Zbynek and Adam, Vojtech and Hrazdilova, Kristyna and Veselý, Radek and Zurek, Ludek and Cihalova, Kristyna},
   article_location = {AUCKLAND},
   article_number = {November 2023},
   doi = {http://dx.doi.org/10.2147/IDR.S435622},
   keywords = {clinical isolates; ampicillin resistance; resistance mechanisms; antibiotics},
   language = {eng},
   issn = {1178-6973},
   journal = {INFECTION AND DRUG RESISTANCE},
   title = {Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps},
   url = {https://www.dovepress.com/escherichia-coli-from-human-wounds-analysis-of-resistance-to--lactams--peer-reviewed-fulltext-article-IDR},
   volume = {16},
   year = {2023}
}

TY  - JOUR
ID  - 2368638
AU  - Rihacek, Martin - Kuthanova, Michaela - Splichal, Zbynek - Adam, Vojtech - Hrazdilova, Kristyna - Veselý, Radek - Zurek, Ludek - Cihalova, Kristyna
PY  - 2023
TI  - Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps
JF  - INFECTION AND DRUG RESISTANCE
VL  - 16
IS  - November 2023
SP  - 7365-7375
EP  - 7365-7375
PB  - DOVE MEDICAL PRESS LTD
SN  - 11786973
KW  - clinical isolates
KW  - ampicillin resistance
KW  - resistance mechanisms
KW  - antibiotics
UR  - https://www.dovepress.com/escherichia-coli-from-human-wounds-analysis-of-resistance-to--lactams--peer-reviewed-fulltext-article-IDR
N2  - Purpose: Resistance of pathogenic strains of Escherichia coli to beta-lactams, particularly to ampicillin, is on the rise and it is attributed to intrinsic and acquired mechanisms. One important factor contributing to resistance, together with primarily resistance mechanisms, is a mutation and/or an over-expression of the intrinsic efflux pumps in the resistance-nodulation-division (RND) superfamily. Among these efflux pumps, AcrA, AcrB, TolC, and AcrD play an important role in antimicrobial co-resistance, including resistance to beta-lactams.Materials and Methods: Twelve E. coli isolates obtained from patients' wounds and the control strain of E. coli ATCC 25922 were analyzed. The phenotypic resistance of these isolates to selected beta-lactams was assessed by determination of the minimal inhibitory concentration. Additionally, the prevalence of beta-lactamase genes (blaTEM, blaCTX-M, blaSHV, and blaAmpC) was screened by PCR. Realtime qPCR was used to determine the expression of the selected efflux pumps acrA, acrB, tolC, and acrD and the repressor acrR after the exposure of E. coli to ampicillin.Results: Phenotypic resistance to beta-lactams was detected in seven isolates, mainly to ampicillin and piperacillin. This was corroborated by the presence of at least one acquired bla gene in each of these isolates. Although E. coli strains varied in the expression of RND-family efflux pumps after the ampicillin exposure, their gene expression indicated that these pumps did not play a major role in the phenotypic resistance to ampicillin.Conclusion: Each E. coli isolate displayed unique characteristics, differing in minimum inhibitory concentration (MIC) values, prevalence of acquired blaTEM and blaCTX-M genes, and expression of the RND-family pumps. This together demonstrates that these clinical isolates employed distinct intrinsic or acquired resistance pathways for their defense against ampicillin. The prevalence and spread of ampicillin resistant E. coli has to be monitored and the search for ampicillin alternatives is needed.
ER  -

RIHACEK, Martin, Michaela KUTHANOVA, Zbynek SPLICHAL, Vojtech ADAM, Kristyna HRAZDILOVA, Radek VESELÝ, Ludek ZUREK and Kristyna CIHALOVA. Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps. \textit{INFECTION AND DRUG RESISTANCE}. AUCKLAND: DOVE MEDICAL PRESS LTD, 2023, vol.~16, November 2023, p.~7365-7375. ISSN~1178-6973. Available from: https://dx.doi.org/10.2147/IDR.S435622.

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