Bafilomycin A1 Molecular Effect on ATPase Activity of
Subcellular Fraction of Human Colorectal Cancer and Rat Liver

J 2024

Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver

BYCHKOVA, Solomiia, Mykola BYCHKOV, Dani DORDEVIC, Monika VÍTĚZOVÁ, Simon K.-M. R. RITTMANN et. al.

Basic information

Original name

Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver

Authors

BYCHKOVA, Solomiia, Mykola BYCHKOV, Dani DORDEVIC, Monika VÍTĚZOVÁ (203 Czech Republic, belonging to the institution), Simon K.-M. R. RITTMANN and Ivan KUSHKEVYCH (203 Czech Republic, belonging to the institution)

Edition

International Journal of Molecular Sciences, MDPI, 2024, 1661-6596

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/ijms25031657

UT WoS

001161210700001

Keywords in English

molecular mechanisms; colon cancer; ATPase; autophagy; hepatocytes; liver; NAADP; biomarkers; bafilomycin A1; Ca2+ store

Abstract

V originále

Bafilomycin A1 inhibits V-type H+ ATPases on the molecular level, which acidifies endo-lysosomes. The main objective of the study was to assess the effect of bafilomycin A1 on Ca2+ content, NAADP-induced Ca2+ release, and ATPase activity in rat hepatocytes and human colon cancer samples. Chlortetracycline (CTC) was used for a quantitative measure of stored calcium in permeabilized rat hepatocytes. ATPase activity was determined by orthophosphate content released after ATP hydrolysis in subcellular post-mitochondrial fraction obtained from rat liver as well as from patients’ samples of colon mucosa and colorectal cancer samples. In rat hepatocytes, bafilomycin A1 decreased stored Ca2+ and prevented the effect of NAADP on stored Ca2+. This effect was dependent on EGTA–Ca2+ buffers in the medium. Bafilomycin A1 significantly increased the activity of Ca2+ ATPases of endoplasmic reticulum (EPR), but not plasma membrane (PM) Ca2+ ATPases in rat liver. Bafilomycin A1 also prevented the effect of NAADP on these pumps. In addition, bafilomycin A1 reduced Na+/K+ ATPase activity and increased basal Mg2+ ATPase activity in the subcellular fraction of rat liver. Concomitant administration of bafilomycin A1 and NAADP enhanced these effects. Bafilomycin A1 increased the activity of the Ca2+ ATPase of EPR in the subcellular fraction of normal human colon mucosa and also in colon cancer tissue samples. In contrast, it decreased Ca2+ ATPase PM activity in samples of normal human colon mucosa and caused no changes in colon cancer. Bafilomycin A1 decreased Na+/K+ ATPase activity and increased basal Mg2+ ATPase activity in normal colon mucosa samples and in human colon cancer samples. It can be concluded that bafilomycin A1 targets NAADP-sensitive acidic Ca2+ stores, effectively modulates ATPase activity, and assumes the link between acidic stores and EPR. Bafilomycin A1 may be useful for cancer therapy.

Links

MUNI/A/1280/2022, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie 3

Investor: Masaryk University, The support of research activities of students of Microbiology 3

MUNI/A/1502/2023, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie 4

Investor: Masaryk University, The support of research activities of students of Microbiology 4

Citovat

BYCHKOVA, Solomiia, Mykola BYCHKOV, Dani DORDEVIC, Monika VÍTĚZOVÁ, Simon K.-M. R. RITTMANN and Ivan KUSHKEVYCH. Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver. International Journal of Molecular Sciences. MDPI, 2024, vol. 25, No 3, p. 1-18. ISSN 1661-6596. Available from: https://dx.doi.org/10.3390/ijms25031657.

@article{2368736,
   author = {Bychkova, Solomiia and Bychkov, Mykola and Dordevic, Dani and Vítězová, Monika and Rittmann, Simon K.andM. R. and Kushkevych, Ivan},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/ijms25031657},
   keywords = {molecular mechanisms; colon cancer; ATPase; autophagy; hepatocytes; liver; NAADP; biomarkers; bafilomycin A1; Ca2+ store},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver},
   url = {https://www.mdpi.com/1422-0067/25/3/1657},
   volume = {25},
   year = {2024}
}

TY  - JOUR
ID  - 2368736
AU  - Bychkova, Solomiia - Bychkov, Mykola - Dordevic, Dani - Vítězová, Monika - Rittmann, Simon K.-M. R. - Kushkevych, Ivan
PY  - 2024
TI  - Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver
JF  - International Journal of Molecular Sciences
VL  - 25
IS  - 3
SP  - 1-18
EP  - 1-18
PB  - MDPI
SN  - 16616596
KW  - molecular mechanisms
KW  - colon cancer
KW  - ATPase
KW  - autophagy
KW  - hepatocytes
KW  - liver
KW  - NAADP
KW  - biomarkers
KW  - bafilomycin A1
KW  - Ca2+ store
UR  - https://www.mdpi.com/1422-0067/25/3/1657
N2  - Bafilomycin A1 inhibits V-type H+ ATPases on the molecular level, which acidifies endo-lysosomes. The main objective of the study was to assess the effect of bafilomycin A1 on Ca2+ content, NAADP-induced Ca2+ release, and ATPase activity in rat hepatocytes and human colon cancer samples. Chlortetracycline (CTC) was used for a quantitative measure of stored calcium in permeabilized rat hepatocytes. ATPase activity was determined by orthophosphate content released after ATP hydrolysis in subcellular post-mitochondrial fraction obtained from rat liver as well as from patients’ samples of colon mucosa and colorectal cancer samples. In rat hepatocytes, bafilomycin A1 decreased stored Ca2+ and prevented the effect of NAADP on stored Ca2+. This effect was dependent on EGTA–Ca2+ buffers in the medium. Bafilomycin A1 significantly increased the activity of Ca2+ ATPases of endoplasmic reticulum (EPR), but not plasma membrane (PM) Ca2+ ATPases in rat liver. Bafilomycin A1 also prevented the effect of NAADP on these pumps. In addition, bafilomycin A1 reduced Na+/K+ ATPase activity and increased basal Mg2+ ATPase activity in the subcellular fraction of rat liver. Concomitant administration of bafilomycin A1 and NAADP enhanced these effects. Bafilomycin A1 increased the activity of the Ca2+ ATPase of EPR in the subcellular fraction of normal human colon mucosa and also in colon cancer tissue samples. In contrast, it decreased Ca2+ ATPase PM activity in samples of normal human colon mucosa and caused no changes in colon cancer. Bafilomycin A1 decreased Na+/K+ ATPase activity and increased basal Mg2+ ATPase activity in normal colon mucosa samples and in human colon cancer samples. It can be concluded that bafilomycin A1 targets NAADP-sensitive acidic Ca2+ stores, effectively modulates ATPase activity, and assumes the link between acidic stores and EPR. Bafilomycin A1 may be useful for cancer therapy.
ER  -

BYCHKOVA, Solomiia, Mykola BYCHKOV, Dani DORDEVIC, Monika VÍTĚZOVÁ, Simon K.-M. R. RITTMANN and Ivan KUSHKEVYCH. Bafilomycin A1 Molecular Effect on ATPase Activity of Subcellular Fraction of Human Colorectal Cancer and Rat Liver. \textit{International Journal of Molecular Sciences}. MDPI, 2024, vol.~25, No~3, p.~1-18. ISSN~1661-6596. Available from: https://dx.doi.org/10.3390/ijms25031657.

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