Preparative agarose gel electrophoresis for reducing matrix
interferences of organoid cell medium prior to LC-MS analysis
of insulin

J 2024

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT et. al.

Basic information

Original name

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

Authors

HRUŠKOVÁ, Helena (203 Czech Republic, belonging to the institution), Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET and Steven Ray WILSON

Edition

Journal of Chromatography A, Elsevier B.V. 2024, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.100 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chroma.2024.464669

UT WoS

001173156700001

Keywords in English

Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids

Abstract

V originále

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.

Citovat

HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET and Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. Journal of Chromatography A. Elsevier B.V., 2024, vol. 1717, February, p. 1-7. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2024.464669.

@article{2369020,
   author = {Hrušková, Helena and Olsen, Christine and Řemínek, Roman and Wang, Chencheng and Aizenshtadt, Aleksandra and Krauss, Stefan and Scholz, Hanne and RøbergandLarsen, Hanne and Foret, František and Wilson, Steven Ray},
   article_number = {February},
   doi = {http://dx.doi.org/10.1016/j.chroma.2024.464669},
   keywords = {Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967324000426},
   volume = {1717},
   year = {2024}
}

TY  - JOUR
ID  - 2369020
AU  - Hrušková, Helena - Olsen, Christine - Řemínek, Roman - Wang, Chencheng - Aizenshtadt, Aleksandra - Krauss, Stefan - Scholz, Hanne - Røberg-Larsen, Hanne - Foret, František - Wilson, Steven Ray
PY  - 2024
TI  - Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
JF  - Journal of Chromatography A
VL  - 1717
IS  - February
SP  - 1-7
EP  - 1-7
PB  - Elsevier B.V.
SN  - 00219673
KW  - Liquid chromatography-mass spectrometry
KW  - Insulin
KW  - Preparative gel electrophoresis
KW  - Stem cell-derived islet organoids
UR  - https://www.sciencedirect.com/science/article/pii/S0021967324000426
N2  - Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.
ER  -

HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET and Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. \textit{Journal of Chromatography A}. Elsevier B.V., 2024, vol.~1717, February, p.~1-7. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2024.464669.

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