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@article{2369020, author = {Hrušková, Helena and Olsen, Christine and Řemínek, Roman and Wang, Chencheng and Aizenshtadt, Aleksandra and Krauss, Stefan and Scholz, Hanne and RøbergandLarsen, Hanne and Foret, František and Wilson, Steven Ray}, article_number = {February}, doi = {http://dx.doi.org/10.1016/j.chroma.2024.464669}, keywords = {Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids}, language = {eng}, issn = {0021-9673}, journal = {Journal of Chromatography A}, title = {Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin}, url = {https://www.sciencedirect.com/science/article/pii/S0021967324000426}, volume = {1717}, year = {2024} }
TY - JOUR ID - 2369020 AU - Hrušková, Helena - Olsen, Christine - Řemínek, Roman - Wang, Chencheng - Aizenshtadt, Aleksandra - Krauss, Stefan - Scholz, Hanne - Røberg-Larsen, Hanne - Foret, František - Wilson, Steven Ray PY - 2024 TI - Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin JF - Journal of Chromatography A VL - 1717 IS - February SP - 1-7 EP - 1-7 PB - Elsevier B.V. SN - 00219673 KW - Liquid chromatography-mass spectrometry KW - Insulin KW - Preparative gel electrophoresis KW - Stem cell-derived islet organoids UR - https://www.sciencedirect.com/science/article/pii/S0021967324000426 N2 - Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin. ER -
HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET and Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. \textit{Journal of Chromatography A}. Elsevier B.V., 2024, vol.~1717, February, p.~1-7. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2024.464669.
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