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@article{2369020,
author = {Hrušková, Helena and Olsen, Christine and Řemínek, Roman and Wang, Chencheng and Aizenshtadt, Aleksandra and Krauss, Stefan and Scholz, Hanne and RøbergandLarsen, Hanne and Foret, František and Wilson, Steven Ray},
article_number = {February},
doi = {http://dx.doi.org/10.1016/j.chroma.2024.464669},
keywords = {Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin},
url = {https://www.sciencedirect.com/science/article/pii/S0021967324000426},
volume = {1717},
year = {2024}
}
TY - JOUR
ID - 2369020
AU - Hrušková, Helena - Olsen, Christine - Řemínek, Roman - Wang, Chencheng - Aizenshtadt, Aleksandra - Krauss, Stefan - Scholz, Hanne - Røberg-Larsen, Hanne - Foret, František - Wilson, Steven Ray
PY - 2024
TI - Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
JF - Journal of Chromatography A
VL - 1717
IS - February
SP - 1-7
EP - 1-7
PB - Elsevier B.V.
SN - 00219673
KW - Liquid chromatography-mass spectrometry
KW - Insulin
KW - Preparative gel electrophoresis
KW - Stem cell-derived islet organoids
UR - https://www.sciencedirect.com/science/article/pii/S0021967324000426
N2 - Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.
ER -
HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET and Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. \textit{Journal of Chromatography A}. Elsevier B.V., 2024, vol.~1717, February, p.~1-7. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2024.464669.
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