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@article{2370317, author = {Silveri, Filippo and Obořilová, Radka and Máčala, Jakub and Compagnone, Dario and Skládal, Petr}, article_location = {Wien}, article_number = {8}, doi = {http://dx.doi.org/10.1007/s00604-023-05873-1}, keywords = {Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion}, language = {eng}, issn = {0026-3672}, journal = {Microchimica Acta}, title = {Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite}, url = {https://link.springer.com/article/10.1007/s00604-023-05873-1}, volume = {190}, year = {2023} }
TY - JOUR ID - 2370317 AU - Silveri, Filippo - Obořilová, Radka - Máčala, Jakub - Compagnone, Dario - Skládal, Petr PY - 2023 TI - Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite JF - Microchimica Acta VL - 190 IS - 8 SP - 1-12 EP - 1-12 PB - Springer SN - 00263672 KW - Human serum albumin KW - Impedimetric sensor KW - Transition metal dichalcogenides KW - Nanocomposite KW - Aqueous-based dispersion UR - https://link.springer.com/article/10.1007/s00604-023-05873-1 N2 - An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers. ER -
SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE and Petr SKLÁDAL. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. \textit{Microchimica Acta}. Wien: Springer, 2023, vol.~190, No~8, p.~1-12. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-023-05873-1.
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