Impedimetric immunosensor for microalbuminuria based on a
WS2/Au water-phase assembled nanocomposite

J 2023

Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite

SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE, Petr SKLÁDAL et. al.

Basic information

Original name

Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite

Authors

SILVERI, Filippo (380 Italy, belonging to the institution), Radka OBOŘILOVÁ (203 Czech Republic, belonging to the institution), Jakub MÁČALA (203 Czech Republic, belonging to the institution), Dario COMPAGNONE and Petr SKLÁDAL (203 Czech Republic, belonging to the institution)

Edition

Microchimica Acta, Wien, Springer, 2023, 0026-3672

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.700 in 2022

RIV identification code

RIV/00216224:14310/23:00133461

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00604-023-05873-1

UT WoS

001033463400005

Keywords in English

Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion

Abstract

V originále

An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.

Links

90242, large research infrastructures

Name: CIISB III

Citovat

SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE and Petr SKLÁDAL. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. Microchimica Acta. Wien: Springer, 2023, vol. 190, No 8, p. 1-12. ISSN 0026-3672. Available from: https://dx.doi.org/10.1007/s00604-023-05873-1.

@article{2370317,
   author = {Silveri, Filippo and Obořilová, Radka and Máčala, Jakub and Compagnone, Dario and Skládal, Petr},
   article_location = {Wien},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s00604-023-05873-1},
   keywords = {Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion},
   language = {eng},
   issn = {0026-3672},
   journal = {Microchimica Acta},
   title = {Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite},
   url = {https://link.springer.com/article/10.1007/s00604-023-05873-1},
   volume = {190},
   year = {2023}
}

TY  - JOUR
ID  - 2370317
AU  - Silveri, Filippo - Obořilová, Radka - Máčala, Jakub - Compagnone, Dario - Skládal, Petr
PY  - 2023
TI  - Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
JF  - Microchimica Acta
VL  - 190
IS  - 8
SP  - 1-12
EP  - 1-12
PB  - Springer
SN  - 00263672
KW  - Human serum albumin
KW  - Impedimetric sensor
KW  - Transition metal dichalcogenides
KW  - Nanocomposite
KW  - Aqueous-based dispersion
UR  - https://link.springer.com/article/10.1007/s00604-023-05873-1
N2  - An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.
ER  -

SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE and Petr SKLÁDAL. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. \textit{Microchimica Acta}. Wien: Springer, 2023, vol.~190, No~8, p.~1-12. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-023-05873-1.

Detailed Information on Publication Record