Detailed Information on Publication Record
2023
Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE, Petr SKLÁDAL et. al.Basic information
Original name
Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
Authors
SILVERI, Filippo (380 Italy, belonging to the institution), Radka OBOŘILOVÁ (203 Czech Republic, belonging to the institution), Jakub MÁČALA (203 Czech Republic, belonging to the institution), Dario COMPAGNONE and Petr SKLÁDAL (203 Czech Republic, belonging to the institution)
Edition
Microchimica Acta, Wien, Springer, 2023, 0026-3672
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Austria
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.700 in 2022
RIV identification code
RIV/00216224:14310/23:00133461
Organization unit
Faculty of Science
UT WoS
001033463400005
Keywords in English
Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion
Tags
International impact, Reviewed
Změněno: 18/10/2024 14:00, Ing. Jana Kuchtová
Abstract
V originále
An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.
Links
| 90242, large research infrastructures |
|