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@inproceedings{2371838, author = {RuttkayandNedecký, Branislav and Sehnal, Karel and Banáš, Dominik and Stankova, Martina and Kepinska, Marta and Bach, Duong Ngonc and Sochor, Jiri and Baron, Mojmir and Farid, Awais and Nguyen, Hoai Viet and Kizek, René}, address = {SLEZSKA}, booktitle = {12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020)}, doi = {http://dx.doi.org/10.37904/nanocon.2020.3746}, editor = {978-80-87294-98-7}, keywords = {Green synthesis; phenolic compounds; analytical methods; antibacterial effects; traditional medicine; ethnobotany}, howpublished = {tištěná verze "print"}, language = {eng}, location = {SLEZSKA}, isbn = {978-80-87294-98-7}, pages = {422-427}, publisher = {TANGER LTD}, title = {PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT}, url = {https://www.confer.cz/nanocon/2020/3746-preparation-of-silver-nanoparticles-prepared-by-green-synthesis-of-vine-seeds-antibacterial-effect}, year = {2021} }
TY - JOUR ID - 2371838 AU - Ruttkay-Nedecký, Branislav - Sehnal, Karel - Banáš, Dominik - Stankova, Martina - Kepinska, Marta - Bach, Duong Ngonc - Sochor, Jiri - Baron, Mojmir - Farid, Awais - Nguyen, Hoai Viet - Kizek, René PY - 2021 TI - PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT PB - TANGER LTD CY - SLEZSKA SN - 9788087294987 KW - Green synthesis KW - phenolic compounds KW - analytical methods KW - antibacterial effects KW - traditional medicine KW - ethnobotany UR - https://www.confer.cz/nanocon/2020/3746-preparation-of-silver-nanoparticles-prepared-by-green-synthesis-of-vine-seeds-antibacterial-effect N2 - Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL. ER -
RUTTKAY-NEDECKÝ, Branislav, Karel SEHNAL, Dominik BANÁŠ, Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN and René KIZEK. PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT. In 978-80-87294-98-7. \textit{12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH \&{}amp; APPLICATION (NANOCON 2020)}. SLEZSKA: TANGER LTD, 2021, p.~422-427. ISBN~978-80-87294-98-7. Available from: https://dx.doi.org/10.37904/nanocon.2020.3746.
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