PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE
SEEDS - ANTIBACTERIAL EFFECT

D 2021

PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

RUTTKAY-NEDECKÝ, Branislav, Karel SEHNAL, Dominik BANÁŠ, Martina STANKOVA, Marta KEPINSKA et. al.

Basic information

Original name

PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

Authors

RUTTKAY-NEDECKÝ, Branislav (203 Czech Republic, belonging to the institution), Karel SEHNAL (203 Czech Republic, belonging to the institution), Dominik BANÁŠ (703 Slovakia, belonging to the institution), Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN and René KIZEK (203 Czech Republic, belonging to the institution)

Edition

SLEZSKA, 12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020), p. 422-427, 6 pp. 2021

Publisher

TANGER LTD

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14160/21:00133494

Organization unit

Faculty of Pharmacy

ISBN

978-80-87294-98-7

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3746

UT WoS

000664505500072

Keywords in English

Green synthesis; phenolic compounds; analytical methods; antibacterial effects; traditional medicine; ethnobotany

Abstract

V originále

Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL.

Citovat

RUTTKAY-NEDECKÝ, Branislav, Karel SEHNAL, Dominik BANÁŠ, Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN and René KIZEK. PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT. In 978-80-87294-98-7. 12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020). SLEZSKA: TANGER LTD, 2021, p. 422-427. ISBN 978-80-87294-98-7. Available from: https://dx.doi.org/10.37904/nanocon.2020.3746.

@inproceedings{2371838,
   author = {RuttkayandNedecký, Branislav and Sehnal, Karel and Banáš, Dominik and Stankova, Martina and Kepinska, Marta and Bach, Duong Ngonc and Sochor, Jiri and Baron, Mojmir and Farid, Awais and Nguyen, Hoai Viet and Kizek, René},
   address = {SLEZSKA},
   booktitle = {12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020)},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3746},
   editor = {978-80-87294-98-7},
   keywords = {Green synthesis; phenolic compounds; analytical methods; antibacterial effects; traditional medicine; ethnobotany},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {SLEZSKA},
   isbn = {978-80-87294-98-7},
   pages = {422-427},
   publisher = {TANGER LTD},
   title = {PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT},
   url = {https://www.confer.cz/nanocon/2020/3746-preparation-of-silver-nanoparticles-prepared-by-green-synthesis-of-vine-seeds-antibacterial-effect},
   year = {2021}
}

TY  - JOUR
ID  - 2371838
AU  - Ruttkay-Nedecký, Branislav - Sehnal, Karel - Banáš, Dominik - Stankova, Martina - Kepinska, Marta - Bach, Duong Ngonc - Sochor, Jiri - Baron, Mojmir - Farid, Awais - Nguyen, Hoai Viet - Kizek, René
PY  - 2021
TI  - PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT
PB  - TANGER LTD
CY  - SLEZSKA
SN  - 9788087294987
KW  - Green synthesis
KW  - phenolic compounds
KW  - analytical methods
KW  - antibacterial effects
KW  - traditional medicine
KW  - ethnobotany
UR  - https://www.confer.cz/nanocon/2020/3746-preparation-of-silver-nanoparticles-prepared-by-green-synthesis-of-vine-seeds-antibacterial-effect
N2  - Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL.
ER  -

RUTTKAY-NEDECKÝ, Branislav, Karel SEHNAL, Dominik BANÁŠ, Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN and René KIZEK. PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT. In 978-80-87294-98-7. \textit{12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH \&{}amp; APPLICATION (NANOCON 2020)}. SLEZSKA: TANGER LTD, 2021, p.~422-427. ISBN~978-80-87294-98-7. Available from: https://dx.doi.org/10.37904/nanocon.2020.3746.

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