RUTTKAY-NEDECKÝ, Branislav, Karel SEHNAL, Dominik BANÁŠ, Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN and René KIZEK. PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT. In 978-80-87294-98-7. 12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020). SLEZSKA: TANGER LTD, 2021, p. 422-427. ISBN 978-80-87294-98-7. Available from: https://dx.doi.org/10.37904/nanocon.2020.3746.
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Basic information
Original name PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT
Authors RUTTKAY-NEDECKÝ, Branislav (203 Czech Republic, belonging to the institution), Karel SEHNAL (203 Czech Republic, belonging to the institution), Dominik BANÁŠ (703 Slovakia, belonging to the institution), Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN and René KIZEK (203 Czech Republic, belonging to the institution).
Edition SLEZSKA, 12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020), p. 422-427, 6 pp. 2021.
Publisher TANGER LTD
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 30104 Pharmacology and pharmacy
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
WWW URL
RIV identification code RIV/00216224:14160/21:00133494
Organization unit Faculty of Pharmacy
ISBN 978-80-87294-98-7
ISSN 2694-930X
Doi http://dx.doi.org/10.37904/nanocon.2020.3746
UT WoS 000664505500072
Keywords in English Green synthesis; phenolic compounds; analytical methods; antibacterial effects; traditional medicine; ethnobotany
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 9/2/2024 09:04.
Abstract
Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL.
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