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@article{2371841,
author = {Bezdekova, Jaroslava and Plevová, Mariana and Nejdl, Lukas and Macka, Mirek and Masařík, Michal and Pacik, Dalibor and Adam, Vojtech and Vaculovicova, Marketa},
article_location = {LAUSANNE},
article_number = {March 2024},
doi = {http://dx.doi.org/10.1016/j.snb.2023.135146},
keywords = {Molecularly imprinted polymers; UV -induced fluorescent clusters; UV -induced fluorescence fingerprinting},
language = {eng},
issn = {0925-4005},
journal = {Sensors and Actuators B: Chemical},
title = {Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device},
url = {https://www.sciencedirect.com/science/article/pii/S0925400523018646?via%3Dihub},
volume = {403},
year = {2024}
}
TY - JOUR
ID - 2371841
AU - Bezdekova, Jaroslava - Plevová, Mariana - Nejdl, Lukas - Macka, Mirek - Masařík, Michal - Pacik, Dalibor - Adam, Vojtech - Vaculovicova, Marketa
PY - 2024
TI - Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device
JF - Sensors and Actuators B: Chemical
VL - 403
IS - March 2024
SP - 1-10
EP - 1-10
PB - Elsevier B.V.
SN - 09254005
KW - Molecularly imprinted polymers
KW - UV -induced fluorescent clusters
KW - UV -induced fluorescence fingerprinting
UR - https://www.sciencedirect.com/science/article/pii/S0925400523018646?via%3Dihub
N2 - The early detection of prostate cancer (CaP), one of the most common cancers in males, improves treatment efficacy. However, current methods to detect CaP are limited by specificity and sensitivity or require an invasive and unpleasant procedure. Therefore, there is a need for a novel, fast, and noninvasive CaP detection method. This proof-of-concept study aimed to diagnose CaP from urine samples by detecting the UV-induced fluorescent clusters formed in situ that reflect a urine composition specific to CaP patients using fluorescence spectroscopy. The UV-induced clusters formed upon irradiation by UV light with a wavelength of 254 nm were detected at excitation and emission wavelengths of 400 nm and 460 nm. Fifteen patients with CaP were correctly distinguished from 5 controls. The different stages of CaP could be further determined using molecular imprinting technology. Additionally, a paper-based analytical device was developed that enabled the correct identification of stage I cancer patients by fluorescent spectroscopy detection and the naked eye. In conclusion, this novel, easyto-operate, point-of-care assay can enable early diagnosis of CaP with high accuracy and judgement of the disease stage. The simplicity, as well as versatility of the proposed method, will enable its application also to other diseases and disorders.
ER -
BEZDEKOVA, Jaroslava, Mariana PLEVOVÁ, Lukas NEJDL, Mirek MACKA, Michal MASAŘÍK, Dalibor PACIK, Vojtech ADAM and Marketa VACULOVICOVA. Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device. \textit{Sensors and Actuators B: Chemical}. LAUSANNE: Elsevier B.V., 2024, vol.~403, March 2024, p.~1-10. ISSN~0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2023.135146.
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