J 2024

Electrochemical HPLC Determination of Piperazine Antihistamine Drugs Employing a Spark-Generated Nickel Oxide Nanoparticle-Modified Carbon Fiber Microelectrode

BELBASI, Zeynab, Jan PETR, Juraj ŠEVČÍK, David JIROVSKY, Jan HRBÁČ et. al.

Basic information

Original name

Electrochemical HPLC Determination of Piperazine Antihistamine Drugs Employing a Spark-Generated Nickel Oxide Nanoparticle-Modified Carbon Fiber Microelectrode

Authors

BELBASI, Zeynab (203 Czech Republic), Jan PETR (203 Czech Republic), Juraj ŠEVČÍK (203 Czech Republic), David JIROVSKY (203 Czech Republic) and Jan HRBÁČ (203 Czech Republic, guarantor, belonging to the institution)

Edition

ACS Omega, WASHINGTON, American Chemical Society, 2024, 2470-1343

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.100 in 2022

Organization unit

Faculty of Science

DOI

UT WoS

001154874700001

Keywords in English

Carbon fiber; Electrodes; High-performance liquid chromatography; Metal oxide nanoparticles; Modification

Tags

Tags

International impact, Reviewed
Změněno: 26/9/2024 14:55, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

In this work, we demonstrate a sensitive high-performance liquid chromatography (HPLC) method for the determination of piperazine antihistamine drugs employing innovative electrochemical detection based on a spark-generated nickel oxide nanoparticle-modified carbon fiber microelectrode built into a miniaturized electrochemical detector. The direct carbon fiber-to-nickel plate electrode spark discharge, carried at 0.8 kV DC, with the nickel electrode connected to the negative pole of the high-voltage power supply, provides extremely fast (1 s) in situ tailoring of the carbon fiber microelectrode surface by nickel oxide nanoparticles. It has been found that nickel oxide nanoparticles exhibit an electrocatalytic effect toward the piperazine moiety electrooxidation process, as confirmed by voltammetric experiments, revealing the shift in the peak potential from 1.25 to 1.09 V versus Ag/AgCl. Cetirizine, cyclizine, chlorcyclizine, flunarizine, meclizine, and buclizine were selected as sample piperazine antihistamine drugs, while diclofenac served as an internal standard. The isocratic reversed-phase separation of the above set was achieved within 15 min using an ARION-CN 3 mu m column with a binary mobile phase consisting of 50 mM phosphate buffer (pH 3) and methanol (45/55, v/v). The limits of detection (LOD) were within the range of 3.8-120 nM (for cyclizine and buclizine) at E = +1500 mV (vs Ag/AgCl), while the response was linear within the concentration range measured up to 5 mu mol L-1. The method was successfully applied to the determination of piperazine antihistamine drugs in spiked plasma samples.