Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study

VYKLICKÁ, Kateřina, Jan BÖHM, Petra BRENEROVÁ, Břetislav LIPOVÝ and Petra BOŘILOVÁ LINHARTOVÁ. Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study. In World of Microbiome. 2023.

Basic information

• Original name: Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study
• Authors: VYKLICKÁ, Kateřina (203 Czech Republic), Jan BÖHM (203 Czech Republic), Petra BRENEROVÁ (203 Czech Republic), Břetislav LIPOVÝ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: World of Microbiome, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Bulgaria
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/23:00133529
• Organization unit: Faculty of Science
• Keywords in English: inhalation injury; airways; respiratory tract; bacteriome; 16S rDNA sequencing

Abstract

Background and Aims Inhalation injury (INHI) is defined as acute airway injury caused by inhalation of hot steam and/or products of combustion. During the hospitalization of these patients, commensal bacterial populations colonizing the lungs, such as Prevotella spp. and Veillonella spp., are displaced by bacteria with pathogenic potential, such as Pseudomonas aeruginosa and Klebsiella pneumonia. In our pilot study, we aimed to determine the dynamic changes of the bacteriome in 10 patients with INHI during their hospitalization. Methods We characterized the bacteriome from oral and oropharyngeal swabs, bronchoalveolar lavage (BAL), catheter urine, and blood using 16S rDNA sequencing at 6 time-points of hospitalization (Days 1 - 28). Results Oral and oropharyngeal swabs revealed high abundance of bacterial DNA. The bacterial composition was quite stable among the studied time-points, and both their alpha- and beta-diversities were similar in similar matrices. The BAL bacteriome composition was related to the oral bacteriome of respective patients; high abundances of Klebsiella sp., Enterobacter sp., Haemophillus sp., Escherichia sp., Staphylococcus sp., Pseudomonas sp., and Neisseria sp. were found in BAL. The results showed that the urine and blood samples were almost sterile at the beginning of the hospitalization; however, in some cases, low amounts of Klebsiella sp. and high abundance of Proteus sp. or Escherichia sp. were found in the blood and urine samples, respectively. Conclusion To conclude, the oral bacteriome seems to be a source of bacterial lung infection in patients with INHI during their hospitalization. Therefore, the oral microbiota could serve as potential a screening marker for the lung microbiome.

Links

• EF17_043/0009632, research and development project: Name: CETOCOEN Excellence
• LM2018121, research and development project: Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

• 857560, interní kód MU(CEP code: EF17_043/0009632): Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)

Investor: European Union, Spreading excellence and widening participation