The effect of intrapartum antibiotics prophylaxis on oral bacteriome in neonates in the first week of theri life - i pilot study

PIVRNCOVÁ, Eliška, Iva KOTÁSKOVÁ, Lucie BUREŠOVÁ, Lenka ANDRÝSKOVÁ, Pavel PILER, Petr JANKŮ, Ivo BOREK, Jana KLÁNOVÁ, Petra VÍDEŇSKÁ, Eva BUDINSKÁ and Petra BOŘILOVÁ LINHARTOVÁ. The effect of intrapartum antibiotics prophylaxis on oral bacteriome in neonates in the first week of theri life - i pilot study. In World of Microbiome. 2023.

Basic information

• Original name: The effect of intrapartum antibiotics prophylaxis on oral bacteriome in neonates in the first week of theri life - i pilot study
• Authors: PIVRNCOVÁ, Eliška (203 Czech Republic), Iva KOTÁSKOVÁ (203 Czech Republic), Lucie BUREŠOVÁ (203 Czech Republic), Lenka ANDRÝSKOVÁ (203 Czech Republic), Pavel PILER (203 Czech Republic), Petr JANKŮ (203 Czech Republic), Ivo BOREK (203 Czech Republic), Jana KLÁNOVÁ (203 Czech Republic), Petra VÍDEŇSKÁ (203 Czech Republic), Eva BUDINSKÁ (703 Slovakia) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: World of Microbiome, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Bulgaria
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/23:00133530
• Organization unit: Faculty of Science
• Keywords in English: oral bacteriome; intrapartum antibiotics; neonates; 16S rDNA sequencing

Abstract

Background: The administration of intrapartum antibiotics prophylaxis (IAP) is commonly used for the prevention of early-onset Group-B-streptococci (GBS) infection. IAP may influence the oral microbiome in neonates. Our study aimed to compare the oral bacteriome between neonates grouped according to their exposure to antibiotics during the delivery and sampling time in the first week of their life. Methods: In this case-control study, oral samples were collected from healthy neonates (n=66) from the CELSPAC:TNG cohort during their hospital stay. The neonates were classified into the group of those delivered by C-section or vaginally with IAP (n=33) and the control group of neonates delivered vaginally without IAP (n=33). The bacteriome profile was determined by 16S rDNA sequencing based on the V3-V4 hyper-variable region. The effect of IAP was tested separately for samples collected within 48 hours of delivery and for those collected later.   Results: Differences in alpha diversity (Shannon index, p=0.01) and bacterial composition (p=0.04, PERMANOVA) were observed in oral samples collected within 48 hours after birth, while bacteriomes in oral samples collected later were similar between IAP and control groups. The genera Streptococcus, Gemella, and Rothia dominated in the oral samples in all groups. Of individual genera, only the relative abundance of Gemella was significantly lower in samples collected within 48 hours after birth in neonates compared to the IAP group (q=0.08). Conclusion: Our results illustrate the effect of IAP on oral bacteriome of neonates within the 48 hours after birth. However, this effect seems to be suppressed during the first week of their life.

Links

• EF17_043/0009632, research and development project: Name: CETOCOEN Excellence
• LM2018132, research and development project: Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR, National Center for Medical Genomics

• LM2018140, research and development project: Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

• LM2023069, research and development project: Name: Výzkumná infrastruktura RECETOX

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure