Other formats:
BibTeX
LaTeX
RIS
@inbook{2374292, author = {Uhrik, Lukas and Henek, Tomas and Planas Iglesias, Joan and Kucera, Josef and Damborský, Jiří and Marek, Martin and Hernychova, Lenka}, address = {New York}, booktitle = {Advanced Methods in Structural Biology}, doi = {http://dx.doi.org/10.1007/978-1-0716-3147-8_18}, editor = {Ângela Sousa, Luis Passarinha}, keywords = {Ancestral luciferase; Hydrogen/deuterium exchange; LoopGrafter; Mass spectrometry; Protein dynamics; Protein engineering}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York}, isbn = {978-1-0716-3146-1}, pages = {293-318}, publisher = {Springer Nature}, title = {Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry}, url = {https://link.springer.com/protocol/10.1007/978-1-0716-3147-8_18}, year = {2023} }
TY - CHAP ID - 2374292 AU - Uhrik, Lukas - Henek, Tomas - Planas Iglesias, Joan - Kucera, Josef - Damborský, Jiří - Marek, Martin - Hernychova, Lenka PY - 2023 TI - Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry VL - 1. PB - Springer Nature CY - New York SN - 9781071631461 KW - Ancestral luciferase KW - Hydrogen/deuterium exchange KW - LoopGrafter KW - Mass spectrometry KW - Protein dynamics KW - Protein engineering UR - https://link.springer.com/protocol/10.1007/978-1-0716-3147-8_18 N2 - Intrinsic protein dynamics contribute to their biological functions. Rational engineering of protein dynamics is extremely challenging with only a handful of successful examples. Hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS) represents a powerful technique for quantitative analysis of protein dynamics. Here we provide a detailed description of the preparation of protein samples, collection of high-quality data, and their in-depth analysis using various computational tools. We illustrate the application of HDX-MS for the study of protein dynamics in the rational engineering of flexible loops in the reconstructed ancestor of haloalkane dehalogenase and Renilla luciferase. These experiments provided unique and valuable data rigorously describing the modification of protein dynamics upon grafting of the loop-helix element. Tips and tricks are provided to stimulate the wider use of HDX-MS to study and engineer protein dynamics. ER -
UHRIK, Lukas, Tomas HENEK, Joan PLANAS IGLESIAS, Josef KUCERA, Jiří DAMBORSKÝ, Martin MAREK and Lenka HERNYCHOVA. Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry. In \^Angela Sousa, Luis Passarinha. \textit{Advanced Methods in Structural Biology}. New York: Springer Nature, 2023, p.~293-318. 1. ISBN~978-1-0716-3146-1. Available from: https://dx.doi.org/10.1007/978-1-0716-3147-8\_{}18.
|