a 2023

Bacteriome analysis of cystic fluids from odontogenic cysts – a pilot study

BOŘILOVÁ LINHARTOVÁ, Petra, Dávid SZÁRAZ, Sabina CERULOVÁ, Lenka BODOKYOVÁ, Ctirad MACHÁČEK et. al.

Basic information

Original name

Bacteriome analysis of cystic fluids from odontogenic cysts – a pilot study

Authors

BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, guarantor, belonging to the institution), Dávid SZÁRAZ (203 Czech Republic), Sabina CERULOVÁ (203 Czech Republic), Lenka BODOKYOVÁ (703 Slovakia), Ctirad MACHÁČEK (203 Czech Republic), Zdeněk DANĚK (203 Czech Republic), Petra BRENEROVÁ (203 Czech Republic) and Eva BUDINSKÁ (703 Slovakia)

Edition

3rd International Conference on Oral Mucosal Immunity and Microbiome, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/23:00133542

Organization unit

Faculty of Science

Keywords in English

bacteriome; cystic fluids; odontogenic cysts
Změněno: 16/2/2024 07:43, Mgr. Terezie Slámová

Abstract

V originále

The diagnosis of developmental odontogenic cyst (OC) is not always clear; it may show inflammatory changes, and in that case the diagnostic features can be lost. As developmental OCs can become secondarily infected, the aim of this pilot study was to investigate bacteriomes of fluids from developmental OCs (e.g. dentigerous cyst, DC; odontogenic keratocyst, OKC) with/without inflammatory changes and compare them with findings in fluids from inflammatory OCs (radicular cyst, RC). Cystic fluids from 38 DCs, 12 OKCs, and 34 RCs were obtained by aspiration using a sterile syringe. Microbial DNA was isolated from cystic fluids and negative controls (N=32) using the QIAamp DNA Mini Kit; isolates were spiked with a mock community. 16S rRNA gene libraries were sequenced on Illumina MiSeq instrument with ≥5000 reads per sample. A sample with a relative abundance of a bacterial genus >20% was considered “positive”. Alpha diversity was significantly decreased in fluids from OCs with inflammation (N=42) in comparison with those without inflammation (N=42; Shannon index, p<0.05). Bacteriomes of fluids from DCs without inflammatory changes were similar to negative controls (p>0.05). Interestingly, 75% of fluids from OKCs were “positive” for DNA of one or more bacterial genera (e.g. Porphyromonas, Prevotella, Parvimonas, Haemophilus). Significant difference in the representation of "positive" samples was observed between those from DC and RC (26% vs. 56% of samples, p<0.05). RC fluids were “positive” for Fusobacterium, Porphyromonas, Prevotella, Fretibacterium, Aggregatibacter, and others. In conclusion, non-sterile cystic fluids from inflammatory DCs, OKCs, and RCs contain DNA from anaerobic periodontal pathogens. These bacteria might play a role in OKCs’ development. In line with our assumption, the content of DCs without inflammatory changes appears to be sterile in most cases.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
LM2023069, research and development project
Name: Výzkumná infrastruktura RECETOX
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure
NU20-08-00205, research and development project
Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst
Investor: Ministry of Health of the CR, Subprogram 1 - standard
857560, interní kód MU
(CEP code: EF17_043/0009632)
Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)
Investor: European Union, Spreading excellence and widening participation