Enrichment of human nasopharyngeal bacteriome with bacteria
from dust after short-term exposure to indoor environment

a 2023

Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment

KLVAŇOVÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ et. al.

Basic information

Original name

Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment

Authors

KLVAŇOVÁ, Eva (203 Czech Republic, belonging to the institution), Petra VÍDEŇSKÁ (203 Czech Republic), Lucie BUREŠOVÁ (203 Czech Republic), Milan URÍK (703 Slovakia), Soňa SMETANOVÁ (203 Czech Republic), Stanislav SMATANA (703 Slovakia), Roman PROKEŠ (203 Czech Republic), Barbara LÁNÍČKOVÁ (203 Czech Republic), Eva BUDINSKÁ (703 Slovakia), Jana KLÁNOVÁ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor)

Edition

Setkání biochemiků a molekulárních biologů, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/23:00133550

Organization unit

Faculty of Science

Keywords in English

bacteriome; dust; nasopharynx; household; hospital; sequencing; 16S rRNA; exposure; indoor environment

Změněno: 16/2/2024 13:05, Mgr. Terezie Slámová

Abstract

V originále

Objective: Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments. Methods: Nasopharyngeal swabs were taken from 22 participants in the morning and after 8 hours of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants’ households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital – NEO, 6 from a pediatric hospital – ENT, and 8 from a research center – RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices. Results: Staphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 hours of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76 % of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These “enriching” bacterial genera overlapped between the hospital workplaces – NEO and ENT but differed from those in the research center – RCX. Conclusions: The results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day, short-term exposure to the indoor environment can result in its enrichment with bacterial DNA from indoor dust, especially from hospitals.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2018132, research and development project

Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR, National Center for Medical Genomics

LM2023069, research and development project

Name: Výzkumná infrastruktura RECETOX

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure

Citovat

KLVAŇOVÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ, Stanislav SMATANA, Roman PROKEŠ, Barbara LÁNÍČKOVÁ, Eva BUDINSKÁ, Jana KLÁNOVÁ and Petra BOŘILOVÁ LINHARTOVÁ. Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment. In Setkání biochemiků a molekulárních biologů. 2023.

@proceedings{2374658,
   author = {Klvaňová, Eva and Vídeňská, Petra and Burešová, Lucie and Urík, Milan and Smetanová, Soňa and Smatana, Stanislav and Prokeš, Roman and Láníčková, Barbara and Budinská, Eva and Klánová, Jana and Bořilová Linhartová, Petra},
   booktitle = {Setkání biochemiků a molekulárních biologů},
   keywords = {bacteriome; dust; nasopharynx; household; hospital; sequencing; 16S rRNA; exposure; indoor environment},
   language = {eng},
   title = {Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment},
   year = {2023}
}

TY  - CONF
ID  - 2374658
AU  - Klvaňová, Eva - Vídeňská, Petra - Burešová, Lucie - Urík, Milan - Smetanová, Soňa - Smatana, Stanislav - Prokeš, Roman - Láníčková, Barbara - Budinská, Eva - Klánová, Jana - Bořilová Linhartová, Petra
PY  - 2023
TI  - Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment
KW  - bacteriome
KW  - dust
KW  - nasopharynx
KW  - household
KW  - hospital
KW  - sequencing
KW  - 16S rRNA
KW  - exposure
KW  - indoor environment
N2  - Objective: Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments. Methods: Nasopharyngeal swabs were taken from 22 participants in the morning and after 8 hours of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants’ households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital – NEO, 6 from a pediatric hospital – ENT, and 8 from a research center – RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices. Results: Staphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 hours of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76 % of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These “enriching” bacterial genera overlapped between the hospital workplaces – NEO and ENT but differed from those in the research center – RCX. Conclusions: The results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day, short-term exposure to the indoor environment can result in its enrichment with bacterial DNA from indoor dust, especially from hospitals.
ER  -

KLVAŇOVÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ, Stanislav SMATANA, Roman PROKEŠ, Barbara LÁNÍČKOVÁ, Eva BUDINSKÁ, Jana KLÁNOVÁ and Petra BOŘILOVÁ LINHARTOVÁ. Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment. In \textit{Setkání biochemiků a molekulárních biologů}. 2023.

Detailed Information on Publication Record