a 2023

The lack of association of selected interleukin 10 polymorphisms with odontogenic cysts in the Czech population

BODOKYOVÁ, Lenka, Dávid SZÁRAZ, Zdeněk DANĚK, Ctirad MACHÁČEK, Petra BOŘILOVÁ LINHARTOVÁ et. al.

Basic information

Original name

The lack of association of selected interleukin 10 polymorphisms with odontogenic cysts in the Czech population

Authors

BODOKYOVÁ, Lenka (703 Slovakia), Dávid SZÁRAZ (203 Czech Republic), Zdeněk DANĚK (203 Czech Republic), Ctirad MACHÁČEK (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Setkání biochemiků a molekulárních biologů, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/23:00133551

Organization unit

Faculty of Science

Keywords in English

interleukin; polymorphisms; odontogenic cysts
Změněno: 16/2/2024 13:11, Mgr. Terezie Slámová

Abstract

V originále

Genetic predispositions play a role in the etiopathogenesis of both inflammatory and developmental odontogenic cysts (OCs). A radicular cyst (RC) is an inflammatory OC, often linked with apical periodontitis (AP) caused by a microbial infection within the root canal system. Dentigerous cysts (DC) and keratocysts (OKC) are the most common developmental OCs; inflammation is also present in some OCs of developmental origin. Another type of neoplasms of odontogenic origin, ameloblastoma (AME), is benign but can be locally aggressive. Previously, an association of aggressive periodontitis with single nucleotide polymorphisms (SNPs) in the interleukin 10, an anti-inflammatory cytokine, was found in the Czech population. Our research aimed to find an association between these IL-10 SNPs and OCs in the Czech population. This genetic association study included 248 patients: 88 with AP without RC, 85 with AP and RC, 53 with DC, 16 with OKC, and 6 with AME. DNA was extracted from peripheral blood leukocytes using the phenol-chloroform method. All three studied SNPs, namely IL-10 −1087 A/G (rs1800896), IL-10 −824 C/T (rs1800871), and IL-10 −597 C/A (rs1800872) are located in the promoter region and may have the ability to modify transcription factor binding sites, thus affecting gene expression. PCR followed by a restriction fragment length polymorphism method (PCR-RFLP) was used for genotyping the SNPs. In our research, the genotype distributions of all studied SNPs in patients were in Hardy-Weinberg equilibrium (p > 0.05); minor allele frequencies were in line with frequencies in the European population. We found no evidence of significant differences between either genotype as well as allele frequencies of the analyzed IL-10 polymorphisms among patient groups with different diagnoses (p > 0.05). In conclusion, we have not found any evidence of association of these IL10 SNPs with the predisposition to both inflammatory and developmental OCs in the Czech population.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
LM2023069, research and development project
Name: Výzkumná infrastruktura RECETOX
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure
NU20-08-00205, research and development project
Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst
Investor: Ministry of Health of the CR, Subprogram 1 - standard
857560, interní kód MU
(CEP code: EF17_043/0009632)
Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)
Investor: European Union, Spreading excellence and widening participation