ISG20L2: an RNA nuclease regulating T cell activation

J 2023

ISG20L2: an RNA nuclease regulating T cell activation

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ, Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ et. al.

Basic information

Original name

ISG20L2: an RNA nuclease regulating T cell activation

Authors

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ (203 Czech Republic, belonging to the institution), Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ (703 Slovakia, belonging to the institution), Julie POKORNÁ (203 Czech Republic, belonging to the institution), Irene FERNANDEZ-DELGADO, Emilio CAMAFEITA, Manuel Jose GOMEZ, Marta RAMIREZ-HUESCA, Cristina GUTIERREZ-VAZQUEZ, Fatima SANCHEZ-CABO, Jesus VAZQUEZ, Štěpánka VAŇÁČOVÁ (203 Czech Republic, belonging to the institution) and Francisco SANCHEZ-MADRID (guarantor)

Edition

Cellular and molecular life sciences, BASEL, BIRKHAUSER VERLAG AG, 2023, 1420-682X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.000 in 2022

RIV identification code

RIV/00216224:14740/23:00133564

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00018-023-04925-2

UT WoS

001058609400002

Keywords in English

Exonuclease; T cell; Immunoregulatory

Abstract

V originále

ISG20L2, a 3' to 5' exoribonuclease previously associated with ribosome biogenesis, is identified here in activated T cells as an enzyme with a preferential affinity for uridylated miRNA substrates. This enzyme is upregulated in T lymphocytes upon TCR and IFN type I stimulation and appears to be involved in regulating T cell function. ISG20L2 silencing leads to an increased basal expression of CD69 and induces greater IL2 secretion. However, ISG20L2 absence impairs CD25 upregula-tion, CD3 synaptic accumulation and MTOC translocation towards the antigen-presenting cell during immune synapsis. Remarkably, ISG20L2 controls the expression of immunoregulatory molecules, such as AHR, NKG2D, CTLA-4, CD137, TIM-3, PD-L1 or PD-1, which show increased levels in ISG20L2 knockout T cells. The dysregulation observed in these key molecules for T cell responses support a role for this exonuclease as a novel RNA-based regulator of T cell function.

Links

GA20-19617S, research and development project

Name: Molekulární mechanismy nekanonických 3' terminálních modifikací RNA a jejich úloha v metabolismu kódujících a nekódujících RNA

Investor: Czech Science Foundation

GA23-07372S, research and development project

Name: Identifikace a úloha proteinů v kontrole a degradaci RNA a regulaci genové exprese

Investor: Czech Science Foundation, Identification and role of proteins in control and degradation of RNA and regulation of gene expression

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ, Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ, Julie POKORNÁ, Irene FERNANDEZ-DELGADO, Emilio CAMAFEITA, Manuel Jose GOMEZ, Marta RAMIREZ-HUESCA, Cristina GUTIERREZ-VAZQUEZ, Fatima SANCHEZ-CABO, Jesus VAZQUEZ, Štěpánka VAŇÁČOVÁ and Francisco SANCHEZ-MADRID. ISG20L2: an RNA nuclease regulating T cell activation. Cellular and molecular life sciences. BASEL: BIRKHAUSER VERLAG AG, 2023, vol. 80, No 9, p. 1-19. ISSN 1420-682X. Available from: https://dx.doi.org/10.1007/s00018-023-04925-2.

@article{2375338,
   author = {RodriguezandGalan, Ana and Dosil, Sara G and Vlčková, Anna and FernandezandMessina, Lola and Feketová, Zuzana and Pokorná, Julie and FernandezandDelgado, Irene and Camafeita, Emilio and Gomez, Manuel Jose and RamirezandHuesca, Marta and GutierrezandVazquez, Cristina and SanchezandCabo, Fatima and Vazquez, Jesus and Vaňáčová, Štěpánka and SanchezandMadrid, Francisco},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.1007/s00018-023-04925-2},
   keywords = {Exonuclease; T cell; Immunoregulatory},
   language = {eng},
   issn = {1420-682X},
   journal = {Cellular and molecular life sciences},
   title = {ISG20L2: an RNA nuclease regulating T cell activation},
   url = {https://link.springer.com/article/10.1007/s00018-023-04925-2},
   volume = {80},
   year = {2023}
}

TY  - JOUR
ID  - 2375338
AU  - Rodriguez-Galan, Ana - Dosil, Sara G - Vlčková, Anna - Fernandez-Messina, Lola - Feketová, Zuzana - Pokorná, Julie - Fernandez-Delgado, Irene - Camafeita, Emilio - Gomez, Manuel Jose - Ramirez-Huesca, Marta - Gutierrez-Vazquez, Cristina - Sanchez-Cabo, Fatima - Vazquez, Jesus - Vaňáčová, Štěpánka - Sanchez-Madrid, Francisco
PY  - 2023
TI  - ISG20L2: an RNA nuclease regulating T cell activation
JF  - Cellular and molecular life sciences
VL  - 80
IS  - 9
SP  - 1-19
EP  - 1-19
PB  - BIRKHAUSER VERLAG AG
SN  - 1420682X
KW  - Exonuclease
KW  - T cell
KW  - Immunoregulatory
UR  - https://link.springer.com/article/10.1007/s00018-023-04925-2
N2  - ISG20L2, a 3' to 5' exoribonuclease previously associated with ribosome biogenesis, is identified here in activated T cells as an enzyme with a preferential affinity for uridylated miRNA substrates. This enzyme is upregulated in T lymphocytes upon TCR and IFN type I stimulation and appears to be involved in regulating T cell function. ISG20L2 silencing leads to an increased basal expression of CD69 and induces greater IL2 secretion. However, ISG20L2 absence impairs CD25 upregula-tion, CD3 synaptic accumulation and MTOC translocation towards the antigen-presenting cell during immune synapsis. Remarkably, ISG20L2 controls the expression of immunoregulatory molecules, such as AHR, NKG2D, CTLA-4, CD137, TIM-3, PD-L1 or PD-1, which show increased levels in ISG20L2 knockout T cells. The dysregulation observed in these key molecules for T cell responses support a role for this exonuclease as a novel RNA-based regulator of T cell function.
ER  -

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ, Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ, Julie POKORNÁ, Irene FERNANDEZ-DELGADO, Emilio CAMAFEITA, Manuel Jose GOMEZ, Marta RAMIREZ-HUESCA, Cristina GUTIERREZ-VAZQUEZ, Fatima SANCHEZ-CABO, Jesus VAZQUEZ, Štěpánka VAŇÁČOVÁ and Francisco SANCHEZ-MADRID. ISG20L2: an RNA nuclease regulating T cell activation. \textit{Cellular and molecular life sciences}. BASEL: BIRKHAUSER VERLAG AG, 2023, vol.~80, No~9, p.~1-19. ISSN~1420-682X. Available from: https://dx.doi.org/10.1007/s00018-023-04925-2.

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