Puncturing lipid membranes: onset of pore formation and the role of hydrogen bonding in the presence of flavonoids

SADZAK, Anja, Zlatko BRKLJACA, Mihael ERAKOVIC, Manfred KRIECHBAUM, Nadica MALTAR-STRMECKI, Jan PŘIBYL and Suzana SEGOTA. Puncturing lipid membranes: onset of pore formation and the role of hydrogen bonding in the presence of flavonoids. JOURNAL OF LIPID RESEARCH. UNITED STATES: ELSEVIER, 2023, vol. 64, No 10, p. 1-14. ISSN 0022-2275. Available from: https://dx.doi.org/10.1016/j.jlr.2023.100430.

Basic information

• Original name: Puncturing lipid membranes: onset of pore formation and the role of hydrogen bonding in the presence of flavonoids
• Authors: SADZAK, Anja, Zlatko BRKLJACA, Mihael ERAKOVIC, Manfred KRIECHBAUM, Nadica MALTAR-STRMECKI, Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Suzana SEGOTA (guarantor).
• Edition: JOURNAL OF LIPID RESEARCH, UNITED STATES, ELSEVIER, 2023, 0022-2275.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 6.500 in 2022
• RIV identification code: RIV/00216224:14740/23:00133566
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.jlr.2023.100430
• UT WoS: 001083841600001
• Keywords in English: Supplementary key words antioxidant; dicarboxylic acid; flavone; flavonol; lipid/peroxidation; oxidized lipid; phospholipid/phosphatidylcholine; physical biochemistry
• Tags: CF NANO, rivok
• Tags: International impact, Reviewed

Abstract

Products of lipid peroxidation induce detrimental structural changes in cell membranes, such as the formation of water pores, which occur in the presence of lipids with partially oxidized chains. However, the influence of another class of products, dicarboxylic acids, is still unclear. These products have greater mobility in the lipid bilayer, which enables their aggregation and the formation of favorable sites for the appearance of pores. Therefore, dodecanedioic acid (DDA) was selected as a model product. Additionally, the influence of several structurally different flavonoids on DDA aggregation via formation of hydrogen bonds with carboxyl groups was investigated. The molecular dynamics of DDA in DOPC lipid bilayer revealed the formation of aggregates extending over the hydrophobic region of the bilayer and increasing its polarity. Consequently, water penetration and the appearance of water wires was observed, representing a new step in the mechanism of pore formation. Furthermore, DDA molecules were found to interact with lipid polar groups, causing them to be buried in the bilayer. The addition of flavonoids to the system disrupted aggregate formation, resulting in the displacement of DDA molecules from the center of the bilayer. The placement of DDA and flavonoids in the lipid bilayer was confirmed by small-angle X-ray scattering. Atomic force microscopy and electron paramagnetic resonance were used to characterize the structural properties. The presence of DDA increased bilayer roughness and decreased the ordering of lipid chains, surface, while flavonoids were found to reduce or reverse these changes.

Links

• LM2018127, research and development project: Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

• 964997, interní kód MU: Name: Alliance for Life Sciences: From Strategies to Actions in Central and Eastern Europe (Acronym: A4L_ACTIONS)

Investor: European Union, Health, demographic change and wellbeing (Societal Challenges)