The workshop was intended for EM facility scientists, managers, and experts from both academia and industry to discuss and share best practices concerning cryo-EM facility operation. The sessions contained presentations on sample preparation and data management strategies. A round table discussion complemented each session to facilitate sharing the experience and brainstorm future directions and needs. The Several topics will be disscussed through the workshop: Advanced robotic platforms for Cryo-EM sample preparation. New strategies to improve sample preparation for cryo-EM (sample purification and time-resolved cryo-EM). Preparation in single particle cryoEM for improved sample quality and time-resolved studies. Delivering more routine sample optimization for cryo-EM with chameleon Cryo-EM data management and real-time processing with Smart EPU and Tomo Live Data management possibilities for Cryo-EM facilities within EOSC. Visit the facilities of EM producers in Brno.